蔬菜粉提取物和β-胡萝卜素对人肺癌细胞增殖和凋亡的影响

目的 :　探讨蔬菜粉脂溶性提取物 ( FEVP)、β-胡萝卜素对人肺癌细胞 YTMLC- 90增殖和凋亡的影响。方法 :　采用体外培养 ,细胞毒 ( MTT法 )试验、形态学、DNA凝胶电泳、流式细胞术等方法。结果 :　高、中、低浓度 FEVP(分别为相当于 β-胡萝卜素 2 .5× 1 0 -6、2 .5× 1 0 -7、5× 1 0 -8mol/L )和高、中浓度β-胡萝卜素 ( 1× 1 0 -4和 1× 1 0 -5mol/L)能显著抑制 YTMLC- 90细胞增殖。高、中浓度 FEVP和高浓度 β-胡萝卜素可显著抑制 YTMLC- 90细胞核分裂。高、中浓度FEVP、高浓度 β-胡萝卜素组细胞出现细胞核固缩 ,染色质凝聚 ;典型 DNA断裂梯状条带 ;细胞凋亡峰。这些现象符合细胞凋亡的特征。结论 :　蔬菜、β-胡萝卜素可能通过抑制细胞核分裂、诱导凋亡抑制人肺癌细胞增殖 ,有剂量反应关系 ,且蔬菜的作用比 β-胡萝卜素的作用强。

EFFECT OF EXTRACTS FROM VEGETABLE POWDER AND β-CAROTENE ON PROLIFERATION AND APOPTOSIS OF HUMAN LUNG CANCER CELLS

Objective: To observe the effects of fat soluble extracts from vegetable powder (FEVP) and β carotene on proliferation and apoptosis of cultured YTMLC 90 human lung cancer cells. Methods: The proliferation was evaluated by MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, DNA content analysis combined with flow cytometric analysis. Results: FEVP was found to inhibit cell proliferation in 2.5×10 -6 mol/L,2.5×10 -7 mol/L, 5×10 -8 mol/L concentration and β carotene was found to inhibit cell proliferation in 1×10 -4 mol/L and 1×10 -5 mol/L concentration. Higher concentration of FEVP and β carotene could inhibit nuclear mitosis and induce morphologic changes consistent with apoptosis in YTMLC 90 cancer cells such as cellular shrinkage, chromatin condensation and nuclear fragmentation, DNA fragmentation ladder, the presence of a sub G 1 apoptotic peak. Conclusion: FEVP inhibits the growth of YTMLC 90 probably via the induction of apoptosis. There is dose dependent relation. Moreover, the effect of FEVP is stronger than that of β carotene.