骨生长因子及骨钙素在新西兰大耳兔体内植入早期镁合金/新骨生成界面的表达变化

目的 观察3种骨生长因子及骨钙素在动物体内植入早期镁合金/新骨生成界面的表达变化. 方法 将具有钙磷涂层的Mg-Mn-Zn合金和对照组裸Mg-Mn-Zn合金分别植入新西兰大耳白兔股骨的上、下干骺端,皮下注射钙黄绿素.在植入1、2、3、4周后分别做X线检查、HE染色、荧光观察和骨形态发生蛋白(BMP)、转化生长因子-β1(TGF-β1)、血小板衍生生长因子(PDGF)、骨钙素(BGP)的免疫组织化学染色. 结果 X线检查见植入4周后的实验组与对照组的合金棒均被新生骨质包裹; HE染色及荧光染色未见实验组与对照组之间有明显差异; 免疫组织化学染色发现,实验组与对照组之间的BMP-2、TGF-β1和PDGF的表达存在明显差异,实验组的表达明显高于对照组. 结论 具有钙磷涂层的Mg-Mn-Zn合金比对照组合金更好地促进骨髓腔内移植物界面处新骨生长因子的表达.

In vivo expression at early stage of bone growth factors and osteocalcin on the magnesium alloy/bone interface in New Zealand white rabbits

Objective To investigate EM>in vivo/EM> expression at early stage of three bone growth factors and osteocalcin on magnesium alloy/bone interface in New Zealand white rabbits. Methods The Ca-P coated and the naked Mg-Mn-Zn alloy rods were implanted respectivelys into the left femoral metaphysis of rabbits. Intravital staining was performed using subcutaneous injections of 1% water solution of calcein to observe the newly formed bone. Postoperatively, three rabbits were sacrificed at 1, 2, 3 and 4 weeks for X-ray examination, fluorescent observation, routine pathological examination and bone morphogenetic protein （BMP）, transforming growth factor βSUB>1/SUB>（TGF-βSUB>1/SUB>）, platelet-derived growth factor （PDGF） and bone gla protein（ BGP） immunohistochemistry. Results X-ray examination showed that both experimental and controlled rods were encircled by new bone, and HE staining and fluorescent observation showed no differences between two groups, but immunohistochemistry and associated statistical ananlysis displayed obvious differences on average absorbance values of BMP-2, TGF-β1 and PDGF expression. Average absorbance values of experimental group expressed higher than thoese of cont