顺铂对肝癌细胞凋亡及其细胞周期的影响

目的：探讨顺铂对Hca-F细胞凋亡及细胞周期的影响。方法：采用荧光，透射电镜及流式细胞术分析法检测肝癌细胞凋亡，细胞周期和增殖指数（PI）的变化。结果：CDDP可引起肿瘤细胞凋亡，并随着给药时间的延长，药物浓度的增高，细胞凋亡率随之增高，细胞凋亡形态结构越发典型，表现为核固缩，染色质边集，核小体形成等，同时药物浓度的升高，细胞周期S期比例下降，G0/G1期比例上升，PI增殖指数下降。结论：CDDP可引起细胞凋亡，细胞周期G0/G1期阻滞，使细胞分裂增殖活动受到抑制。

CDDP result in Cell cycle and Induction of Apoptosis in Hca-F cell line

Objective:To observe the correlation between CDDP and Hca F cell lines in the qualitative and quantitative of apoptosis,cell cycle and proliferation index.Methods:The staining of Hoechst 33342 and PI fluorecence,transmission electron microscopic technique and flow cytometry(FCM)were used to detect the above results.Results:CDDP could induce Hca F cell apoptosis markedly.The ratio of apoptosis would increase with time lengthening and the concentration of CDDP,and would appear PI and S phase decreasing as well as G 0/G 1 phase increasing.Conclusion:CDDP can induce Hca F cell apoptosis,arrest cell cycle and inhibit cell proliferation.