甘氨脱氧胆酸诱导大鼠肝细胞凋亡

目的 探讨甘氨脱氧胆酸（Ｇｌｙｃｏｄｅｏｘｙｃｈｏｌａｔｅ,ＧＤＣ）与大鼠肝细胞凋亡的关系。方法 以ＧＤＣ为处理因素,体外培养大鼠原代肝细胞,分别用光镜、电镜、ＴＵＮＥＬ原位杂交,流式细胞术和ＤＮＡ电泳技术观察和分析ＧＤＣ诱大鼠肝细胞凋亡的作用。结果 大鼠肝细胞在加入终浓度为１００μｍｏｌ／Ｌ的ＧＤＣ培养２ｈ后,光镜、电镜、ＴＵＮＥＬ原位杂交,可见凋亡细胞,终浓度为５０、１００、１５０μｍｏｌ／Ｌ

Rat hepatocellular apoptosis induced by glycodeoxycholate

OBJECTIVE: To explore the relationship between glycodeoxycholate (GDC) and rat hepatocellular apoptosis. METHODS: GDC was used to treat rat hepatocytes cultured in vitro and the apoptotic cells were observed and analyzed with light microscope, transmission electron microscope, TUNEL in situ hybridization, DNA agarose gel electrophoresis and flow cytometry. RESULTS: When rat hepatocytes were incubated with GDC (final concentration of 100 micromol/L) for 2h, apoptotic hepatocytes were observed with light and transmission electron microscope, and TUNEL in situ hybridization. When the hepatocytes were incubated with GDC (final concentration of 50, 100, 150 micromol/L, respectively) for 2h, or with GDC (final concentration of 100 micromol/L) for 2, 4, 6, 8h, respectively, agarose gel electrophoresis of the hepatocytes DNA demonstrated the typical ladder patterns. CONCLUSION: GDC with final concentration of 50, 100, 150 micromol/L could induce rat hepatocellular apoptosis.