电化学免疫传感器超灵敏检测髓过氧化物酶的研究

目的：本研究拟建立一种电化学免疫传感器用于超灵敏检测人血清中髓过氧化物酶（MPO）水平的方法。方法通过石墨化多孔碳-金复合纳米材料（AuNPs＠ GMCs）将 MPO 抗体固定到玻碳电极表面，研制出一种新型 MPO 电化学免疫传感器，探讨了实验条件对传感器性能的影响，并与酶联免疫吸附试验（ELISA）进行方法学比较。结果在最优条件下该传感器对MPO 反应灵敏，其线性范围为2．000～300．000 ng／mL ，相关系数为0．999，检出限为0．5 ng／mL ，与 ELISA 法检测结果的相关系数为0．983。结论该电化学免疫传感器可实现对 MPO 的超灵敏检测。

Study on the electrochemical immunosensor for ultrasensitive detection of myeloperoxidase

Objective To construct an electrochemical immunosensor for ultrasensitive determination of myeloperoxidase (MPO) .Methods The electrochemical immunosensor for M PO was prepared by modifying the electrode using Au-graphitized me-soporous carbon nanoparticles(AuNPs@ GMCs) hybrid and immobilizing MPO antibodies onto the glass carbon electrode surface . The effect of experimental parameters on the immunosensor and results comparison with ELISA were investigated .Results The immunosensor was sensitive to M PO with a linear relationship between 2 .000 and 300 .000 ng/mL and a correlation coefficient of 0 .999 ;the detection limit was 0 .5 ng/mL .The correlation coefficient of two methods was 0 .983 .Conclusion The immunosensor can be used for ultrasensitive detection of MPO .