缺氧对人牙周膜细胞OPG和RANKL 基因表达的影响

目的：研究缺氧对人牙周膜细胞骨保护素（OPG）和核因子κb受体活化因子配体（RANKL）基因表达的影响，揭示缺氧在正畸牙移动压力侧骨吸收中的作用。方法采取酶消化法结合组织块法培养人牙周膜细胞。取3～5代细胞，分别在常氧（O2浓度为20％，对照组）和缺氧（O2浓度为2％）状态下培养3、6、12、24 h ，采用RT‐PCR检测OPG、RANKL mRNA的表达情况。采用SPSS15．0软件包，对RT‐PCR数据进行单因素方差分析。结果在培养3、6 h时，缺氧组和对照组OPG、RANKL mR‐NA的表达水平比较差异无统计学意义（P＞0．05）；在培养12、24 h时，缺氧组OPG mRNA 表达水平低于常氧组，而缺氧组RANKL mRNA的表达水平高于常氧组，缺氧组RANKL／OPG的比值较常氧组升高，差异有统计学意义（P＜0．05）。结论缺氧可以影响人牙周膜细胞OPG、RANKL mRNA的表达，在正畸牙移动压力侧骨吸收中起促进作用。

Regulation of mRNA expression of OPG and RANKL in hPDLCs by hypoxia in vitro

Objective To study the effect of hypoxia on the mRNA expression of osteoprotegerin(OPG) and receptor activa‐tor for nuclear factor‐κB ligand(RANKL) in human periodontal ligament cells(hPDLCs) and to explore the role of hypoxia in the orthodontic bone resorption in pressure side .Methods The primary hPDLCs were cultivated with enzyme digestion assay and tis‐sue cultivation .The 3-5 generations of hPDLCs were respectively cultured 3 ,6 ,12 and 24 h in normoxia condition (20% O2 ) or in hypoxia condition (2% O2 ) .The mRNA expression of OPG and RANKL were detected with RT‐PCR .The experimental data was analyzed by one way ANOVA using SPSS15 .0 .Results After cultivated in hypoxia condition for 3 h or 6 h ,the mRNA expression of OPG and RANKL in hPDLCs didn′t change significant(P> 0 .05) .After cultivated in hypoxia condition for 12 h or 24 h ,the mRNA expression of OPG in hPDLCs decreased while the RANKL increased .Thus the ratio of RANKL/OPG increased and the difference was significant(P<0 .05) .Conclusion Hypoxia can regulate the mRNA expression of OPG and RANKL in hPDLCs and will promote the orthodontic bone resorption in pressure side .