不同炮制方法对连翘中连翘苷含量的影响

目的：比较不同炮制方法对连翘中连翘苷含量的影响。方法：在色谱柱为美国wat ers symmet rycol umns（250 mm×4.6 mm,5μm）;流动相为甲醇-水,程序梯度洗脱;流速为1.0 mL/mi n;检测波长为280 nm;进样量为20μL;柱温：25℃的条件下采用高效液相色谱法测定连翘蒸晒品、生晒品中连翘苷的含量及峰面积,并与连翘苷对照品进行对比;结果：连翘果实蒸、生晒品所含成分几乎没发生变化,但在含量上前者明显高于后者。结论：连翘在炮制过程中蒸晒、生晒对其有效成分组成影响不大,但蒸晒品连翘苷的含量较好,这充分说明连翘蒸品优于生品。

The Effect of Different Processing Methods on Glycosides Content in Forsythia

Objective：To compare the different processing methods on forsythia glycosides content.Method：The column was from the United States waters symmetry columns（250 mm×4.6 mm,5 μm）;mobile phase was methanol-water,gradient elution program;flow rate of 1.0 mL/min;detection wavelength was 280 nm;injection volume 20 μL;column temperature：25℃,which were the conditions of high performance liquid chromatography forsythia steam drying products,raw materials in the drying phillyrin content and peak area,and compared with the reference substance phillyrin.result：Forsythia steamed fruit,Sun-dried product contains almost no change in composion,but significantly higher than in the content of the latter on the former.Conclusion：Forsythia in the process of steam drying processing,Sun-dried little effect of its active ingredients,but the steam drying products Forsythin content was better,that showed it better than the raw materials forsythia steam products.