淫羊藿苷延长秀丽线虫寿命与机制

目的探讨淫羊藿苷（Icariin，Ica）对秀丽线虫寿命的干预效果与机制，为筛选延缓衰老、延长寿命的药物提供实验依据。方法将秀丽线虫分为空白对照组、Ica5mg／L、10mg／L、15mg／L、20mg／L各剂量干预实验组，观察在Ica不同梯度剂量作用下秀丽线虫寿命的变化，以明确Ica作用的最佳剂量。接着设立秀丽线虫空白对照组和Ica（15mg／L）干预组，评价Ica对秀丽线虫平均寿命、生殖能力的干预效果；同时进行急性热应激和急性氧化应激实验，观察应激时线虫的平均生存时间，探讨Ica延长线虫寿命的机制。结果15mg／L的Ica是延长秀丽线虫寿命的最佳剂量。Ica组线虫的平均寿命明显长于空白对照组[（31．87±4．46）d vs．（20．13±2．81）d]（P〈0．01）；Ica组线虫生殖高峰期子代数目比空白对照组显著增加（151±21．13VS．122±17．09，P〈O．01）；急性热应激实验和急性氧化应激实验发现Ica组线虫平均生存时间均明显长于空白对照组[（9．53±1．34）hVS．（5．97±0．84）h，（8．34±1．17）hvs.（5．77±O．81）h]（P〈0．01）。结论淫羊藿苷能延长秀丽线虫的寿命，其机制可能与淫羊藿苷提高线虫的应激能力有关。

Effect of icariin on lifespan of Caenorhabditis elegans

Objective To evaluate the effect of Icariin （Ica） on lifespan of C. elegans in order to screen drugs for longevity. Methods （1） Dosage-effect test： C elegans, which were treated with Ica 0 mg/L, Ica 5 rag/L, 10 mg/L, 15 mg/L, 20 mg/L, respectively, were tested by lifespan assay to determine the best effective dosages of Ica. （2） Lifespan assay： C. elegans were divided into control group and Ica （15mg/L） treatment group. Lifespan assay was used to evaluate the life-extension effect of Ica on C elegans. （3） Fecundity test： C elegans fecundity in the Ica （15mg/L） treatment and control groups was assessed by evaluating the effect of Ica on the worms＇ reproduction. （4） Stress resistance test： the lifespan of C elegans in the Ica （15 mg/L） treatment and control groups were assessed by acute heat and oxidative stress treatment to explore the initial mechanisms involved in the effect of Ica on lifespan. Results 15 mg/L of Ica was the best dosage to made C elegans get the maximum lifespan. The average lifespan of C. elegans in the Ica-treated group was sig- nificantly longer than the control group [（31.87±4.46） d vs. （20.13±2.81） d ] （P〈0.01）. Compared with the control group, the C. elegans worms＇ reproduction of the Ica-treated group was significantly enforced （ 151 ± 21.13 vs. 122 ± 17.09 ） （P〈0.01）. Ica significantly extended the average survival time of C. elegans as compared with the control group under the acute heat and oxidative stress conditions [（9.53± 1.34） h vs. （5.97±0.84） h, （8.34± 1.17） h vs. （5.77±0.81） h] （P 〈 0.01 ）. Conclusion Icariin has a good effect on anti-aging and longevity of C elegans and the mechanism may involve in the improvement of heat and oxidative stress resistance ability of C elegans.