干扰素对丝裂霉素C诱发的姊妹染色单体互换和紫外线诱发的DNA修复合成影响的研究

本文报告了关于干扰素对丝裂霉素C诱发的姊妹染色单体互换频率和(或)对紫外线诱发的非合成期DNA合成的影响的研究。结果发现,4例正常人和3例着色性干皮病病人的淋巴细胞经干扰素处理后再用丝裂霉素C所诱发的姊妹染色单体互换频率显著地低于对照组,而2例正常人和2例着色性干皮病病人的淋巴细胞经干扰素处理后再用紫外线所诱发的非合成期DNA合成水平则与对照组相似。作者认为,干素扰可能具有抗变效应。

EFFECTS OF INTERFERON ON MITOMYCIN-INDUCED SISTER CHROMATID EXCHANGES AND ON ULTRAVIOLET-LIGHT-INDUCED UNSCHEDULED DNA SYNE THESIS

We studied the effects of interferon(IFN) on sister chromatid exchanges(SCE) induced by mitomycin(MMC) and/or on unscheduled DNA synthesis(UDS ) induced by ultraviolet light(UV) in peripheral lymphocytes taken from 4 normal individuals and 3 patients with xeroderma pigmentosum(XP) in whom the existence of congenital DNA excision repair defect had been verified.The lymphocytes from 2 normal individuals and 3 patients with XP were treated with IFN 8 hours before MMC was added SCE frequencies in IFN groups were lower than those in the controls. Statistical computation showed that all differences were highiy significant(P<0.01).Measurement of UDS was undertaken by the method of Burk et al. (1971).The lymphocytes from 2 normal individuals and 2 patients with XP were treated with IFN 8 hours before MMC was added. Ultraviolet-induced 3H-TdR incorporation in the IFN group was similar to that of the control in all cases. No significant differences exist between the different groups in each individual(P>0.05).