Cell size-specific appearance of neuropeptide Y in the trigeminal ganglion following peripheral axotomy of different branches of the mandibular nerve of the rat |
| |
Authors: | S. Wakisaka S. Takikita Y. Sasaki J. Kato M.J. Tabata K. Kurisu |
| |
Affiliation: | aFirst Department of Oral Anatomy, Osaka University Faculty of Dentistry, Osaka, Japan |
| |
Abstract: | The effect of peripheral axotomy of the mental nerve (MN) and the cutaneous branch of the mylohyoid nerve (MhN) on the appearance of neuropeptide Y-like immunoreactivity (NPY-IR) in cells in the trigeminal ganglion of the rat was examined with combined retrograde-tracing and immunohistochemistry. Retrograde-tracing with True Blue (TB) revealed that the cell-size spectrum of the trigeminal cells sending peripheral processes to the MN (TB MN cells) ranged from 75.9 to 1560.5 μm2 (or from 9.8 to 44.6 μm in diameter); 53% of TB MN cells were 300–600 μm2. TB MhN cells ranged from 47.7 to 1261.5 μm2 (or from 7.8 to 40.1 μm in diameter); 56% of TB MhN cells were <300 μm2. In the normal trigeminal ganglion, there were no NPY-IR cells. 14 days after MN transection, 35% of TB MN cells displayed NPY-IR. The distribution of the cross-sectional areas of NPY-IR cells after MN transection was very similar to that of TB MN cells. Transection of MhN also induced the appearance of NPY-IR in the trigeminal ganglion but to a lesser extent (17% of TB MhN cells). The distribution of the cross-sectional areas of NPY-IR cells after MhN transection was similar to that of NPY-IR cells after MN transection. These results indicate that injury-evoked NPY-IR is specific for the medium- and large-sized ganglion cells. |
| |
Keywords: | Neuropeptide Y Axotomy Mental nerve Mylohyoid nerve Trigeminal ganglion |
本文献已被 ScienceDirect 等数据库收录! |