Characterization of polysaccharides and their antioxidant properties from Plumula nelumbinis |
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| Authors: | Yueping Jiang Wen Zi Zhifang Pei Shao Liu |
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| Affiliation: | 1. Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China;2. Institute of Hospital Pharmacy, Central South University, Changsha 410008, China;3. Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China |
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| Abstract: | Two novel polysaccharides, Plumula nelumbinis (P. nelumbinis) polysaccharide I (LNP I) and P. nelumbinis polysaccharide II (LNP II), were extracted and purified from P. nelumbinis, and a sulfated polysaccharide, P. nelumbinis polysaccharide III (LNP III), with a substitution degree of 0.62 was prepared from LNPI. The structures of the LNPs were preliminarily characterized using high performance size exclusion chromatography (HPSEC), gas chromatography-mass spectrometry (GC–MS), Fourier transformed infrared spectrometry (FT-IR), and nuclear magnetic resonance (NMR) spectrometry. In addition, evaluation of the antioxidant activity of the LNPs showed that they could significantly increase the proliferation of RAW264.7 macrophages (P?2O2-induced oxidative damage. This suggested that these LNPs may be used as potential antioxidants. |
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| Keywords: | Polysaccharides Sulfated modification Antioxidant activity |
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