Vitamin B12 protects against DNA damage induced by hydrochlorothiazide |
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Authors: | Karem H Alzoubi Erva Bayraktar Omar Khabour Sayer I Al-Azzam |
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Institution: | 1. Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan;2. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan |
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Abstract: | DNA damage induced by hydrochlorothiazide was previously reported in cultured human lymphocytes. In this study, we aimed to investigate the harmful effects of hydrochlorothiazide on DNA by measuring the spontaneous frequency of sister chromatid exchanges (SCEs) in cultured human lymphocytes. We also aimed to investigate the possible protection of that damage by vitamin B12. The results showed that hydrochlorothiazide (5?µg/mL) significantly increased the frequency of sister chromatid exchanges (P?<?0.001) in human lymphocytes in comparison with control. Additionally, the frequency of hydrochlorothiazide-induced SCEs was significantly decreased by co-treatment with vitamin B12 at concentration of 13.5?µg/mL (P?<?0.001). In conclusion, hydrochlorothiazide is genotoxic to human lymphocytes and its toxicity is reduced by vitamin B12. |
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Keywords: | Vitamin B12 Hydrochlorothiazide DNA damage Sister chromatids exchange Genotoxicity |
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