某院宫颈疾病患者人乳头瘤病毒感染及分型情况

[目的]应用流式荧光杂交法检测人乳头瘤病毒(HPV),分析宫颈疾病患者HPV感染及分型情况。[方法]以2013年1月至2013年12月浙江省宁海县第一医院4 041例宫颈疾病患者作为研究对象,采集宫颈脱落细胞,用流式荧光杂交技术检测HPV基因型,并对HPV感染率、基因亚型、感染类型和年龄分布等进行分析。[结果]检出HPV感染744例(18.41%),其中单一亚型感染603例(14.92%),多重亚型感染141例(3.49%)。可供检测的26种HPV基因亚型均有检出,最常见的亚型为HPV-16、52、58、39、18、61、59、6等,其中低危型以HPV-61、6、11亚型感染为主。高危型以HPV-16、52、58亚型感染为主。HPV感染亚型以高危亚型为主,单一高危亚型感染阳性率13.07%(528/4 041),多重高危亚型感染阳性率2.15%(87/4 041)。31~40岁妇女HPV感染阳性率最高,阳性率22.22%,50岁以上者HPV感染阳性率最低,阳性率13.99%,且各年龄组中均以高危亚型感染检出率最高。[结论]宫颈疾病患者中HPV感染率较高,基因型分布较广,流式荧光杂交法是检测HPV感染的快速有效方法,HPV分型检测对宫颈疾病的防治意义重大。

Human papillomavirus infection and typing situation in cervical disease patients in a hospital

[Objective] To investigate HPV infection and its typing situation in cervical disease patients in a hospital by flow fluorescence hybridization for detection of human papillomavirus (HPV). [Methods] From January to December 2013, a total of 4 041 cervical disease patients were as research objects. The cervical exfoliated cell specimens were collected and HPV genotypes were detected by flow fluorescence hybridization, while HPV infection rate, genetic subtypes, infection type and age distribution were analyzed. [Results] HPV infection was found in 744 cases(18.41%), in which 603 cases(14.92%) were single subtype and 141 cases(3.49%) multiple subtypes of infection. All 26 kinds of HPV subtypes available to detection were detected. The common subtypes were HPV-16, 52,58,39,18,61,59,6 etc., in which HPV-61,6,11 subtypes were with low-risk and HPV-16,52,58 subtypes with high-risk. More common type of infection was high-risk subtype, in which single high-risk subtype infection was 13.07%(528/4 041) and multiple high-risk subtypes infection 2.15%(87/4 041).The highest rate of HPV infection(22.22%) was found in the women 31 to 40 years of age, and the lowest(13.99%) in those above 50 years.In all age groups, the high-risk subtype infection was found with the highest detection rate. [Conclusion] Patients with cervical disease are found to have higher HPV infection rate with wider distribution genotype. Flow fluorescence hybridization proves a fast and effective method to detect HPV infection, and HPV genotyping has significance for prevention and treatment of cervical disease.