2-甲氧基雌二醇诱导骨髓瘤细胞系CZ-1细胞分化作用初探

目的　观察2 甲氧基雌二醇对骨髓瘤细胞系CZ 1细胞诱导分化作用。方法　通过细胞形态观察,细胞表面标志分析和细胞分泌轻链蛋白水平的测定,观察2 甲氧基雌二醇对CZ 1细胞的诱导分化作用。结果　CZ 1细胞经0. 1~0. 5μmol/L2 甲氧基雌二醇作用48h后细胞形态向成熟阶段发展,表现为细胞胞核缩小,胞浆丰富,核浆比例下降,核仁减少或消失,核染色质变粗、变密。细胞表面标志CD49e阳性表达率由(12. 20±1. 57)%增加到( 24. 80±1. 26 )%,差异有统计学意义(P<0. 05); CZ 1细胞分泌轻链蛋白由(35. 97±2. 60)μg/ml升高到(79. 67±1. 88)μg/ml,差异有统计学意义(P<0. 05)。结论　较低浓度2 甲氧基雌二醇可诱导骨髓瘤细胞向成熟阶段分化。

Effect of 2-methoxyestradiol on cell differentiation of myeloma cell line CZ-1

OBJECTIVE: To investigate the differentiation induction effect of 2-methoxyestradiol (2ME2), an estrogen derivative on myeloma cell line CZ-1. METHODS: The changes of CZ-1 cells in morphology, expression of surface CD49e and quantity of light chain secretion in the supernatant were observed when treated with 0.1 approximately 0.5 micromol/L 2ME2 for 48 h. RESULTS: 2ME2 could induce differentiation of CZ-1 cells. The cells appeared decreased in size of nucleus, increased in cytoplasma, decreased in the ratio of nucleus to plasma, decreased in number or disappearance of nucleolus, and thickness and pyknosis of chromatin. The expression of CD49e was increased from (12.20 +/- 1.57)% to (24.80 +/- 1.26)% (P < 0.05). Light chain secretion in the supernatant was increased from (35.97 +/- 2.60) microg/ml to (79.67 +/- 1.88) microg/ml (P < 0.05). CONCLUSION: Low concentrations of 2ME2 could induce differentiation of myeloma cell line CZ-1.