大肠癌组织中凋亡抑制蛋白survivin的表达及其与bcl-2、c-erbB2表达的关系

目的 :探讨凋亡相关基因survivin在大肠癌组织中的表达与临床病理及与bcl 2和c erbB2表达的关系。方法 :采用免疫组化的方法检测 12例大肠正常粘膜及 5 7例大肠癌患者肿瘤组织中survivin和bcl 2、c erbB2的表达。结果 :12例大肠正常粘膜中survivin呈阴性表达 ,阳性率 0 0 % ;5 7例大肠癌组织中survivin阳性表达 3 6例 ,阳性率 63 2 % ;bcl 2阳性表达 3 3例 ,阳性率 5 7 9% ;c erbB2阳性表达 3 0例 ,阳性率 5 2 6%。survivin表达与患者年龄、性别、组织分型、淋巴结转移及Dukes分期等无明显相关性。survivin在bcl 2阳性和阴性患者中阳性率分别为 72 7% ( 2 4/3 3 )和 2 9 2 % ( 7/2 4) ,两者间有显著差异 (P <0 0 1) ;survivin在c erbB2阳性和阴性患者中阳性率分别为 73 3 % ( 2 2 /3 0 )和 3 7 0 % ( 10 /2 7) ,两者间也有显著差异 (P <0 0 1)。结论 :大肠癌患者survivin高表达导致的凋亡抑制在大肠癌的发生发展中起了重要作用 ,survivin表达与大肠癌中bcl 2和c erbB2异常表达相关。

Expression of antiapoptosis protein,survivin,correlated with the expression of bcl-2 and c-erbB2 in colorectal carcinoma

Objective: To study the expression of survivin, an inhibitor of apoptosis protein, in colorectal carcinomas and its relationship with the expression of bcl-2 and c-erbB2.Methods: Using immunohistochemical staining method, we examined the expression of survivin, bcl-2 and c-erbB2 genes in 12 cases of normal colorectal mucosas and 57 cases of colorectal carcinomas.Results: No immunoreactivity of survivin was seen in normal colorectal mucosas. Expression of survivin was observed in the cytoplasm of colorectal carcinoma cells and survivin expressed in 36 of 57(63.2%) cases of colorectal carcinomas. Overexpression of survivin had no relation with age, gender, histological type, lymph node metastasis, and Dukes classification .But the expression of survivin significantly associated with bcl-2 expression(24/33, 72.7% versus 7/24, 29.2%, P<0.01) and c-erbB2 expression(22/30, 73.7% versus 10/27, 37.0%, P<0.01).Conclusion: These data indicated that survivin play an important role in the onset of colorectal carcinoma and suggested potential correlation between survivin and bcl-2, and between survivin and c-erbB2 as well.