尿脱落细胞HPV16/18 E7基因表达对膀胱移行细胞癌诊断价值的研究

目的：探讨尿脱落细胞高危型人乳头状瘤病毒16、18E_7基因(HPV_(16/18)E_7)在膀胱移行细胞癌中的表达。方法：利用聚合酶链反应(Polymerase chain reaction,PCR)和特异性核酸内切酶技术对60例膀胱移行细胞癌患者的尿液标本进行研究。结果：膀胱移行细胞癌Ⅰ级、Ⅱ级和Ⅲ级HPV_(16)的检出率分别为73．7%、78．5%和92．3%,总的检出率为80．0%(48/60)；HPV_(18)检出率分别为73．7%、71．4%和84．6%,总的检出率为75．0% (45/60)。由此可知,HPV_(16/18)的检出率差别无统计学意义(P＞0．01),但膀胱移行细胞癌Ⅲ级的检出率最高(P＜0．05)。结论：尿液中HPV_(16/18)E_7基因的检测可作为膀胱癌早期诊断的一种方法。

A diagnosis value study of the gene expression of human papillomavirus (HPV) 16/18 E7 in urine cast-off cells of transitional cell bladder cancer

Objective To investigate the gene expression of human papillomavirus(HPV)16/18 E_7 in urine cast-off cells of transitional cell bladder cancer.Methods:60 sample of urine cast-off ceils of transitional cell bladder cancer had been detected by the polymerase chain reaction(PCR)using special primer and DNA restriction endo- nuclease technique(DNA RET).Results:Of 60 transitional cell bladder cancer,80.0%(48/60)were positive gene expression for HPV_(16) DNA(among them,73.3% for one grade transitional cell bladder cancer,78.50% for,two grade transitional cell bladder cancer 92.3% three grade transitional cell biadder cancer)and 75%(45/60)were positive gene expression for HPV_(18) DNA(among them,one,two and three grade transitional cell bladder cancer were 46.7%,66.7% and 44.3% respectively).Conclusions:Our data suggest that human papillomavirus is unlike- ly to be involved in the pathogenesis of transitional cell carcinoma,it is probably a earlier diagnosis methods in transitional cell carcinoma by detecting HPV_(16/18)E_7 in urine cast-off cells of transitional cell bladder cancer.