当归注射液对免疫诱导再生障碍性贫血小鼠骨髓细胞组织形态及超微结构的影响

目的　探讨当归注射液对免疫诱导再生障碍性贫血 (AA)小鼠骨髓细胞组织形态及超微结构的影响。方法　雌性Balb/c小鼠 ,随机分为正常组、模型组、治疗组和对照组。于d1 2每只小鼠采集尾静脉血测外周血白细胞计数 ,后断颈处死小鼠 ,取出尺骨及股骨 ,计数骨髓单个核细胞。并行尺骨组织学切片及超微结构观察。结果　与正常组比较 ,模型组造血细胞明显减少 ,骨髓微环境受损 ,治疗组能明显改善骨髓增生程度 ,修复骨髓微环境 ,增加骨髓单个核细胞计数 ;与对照组比较无显著差异。结论　当归注射液能通过促进骨髓造血细胞增生 ,改善骨髓微环境对免疫诱导AA小鼠进行保护作用。

Influence of Angelica Sinenisis Injection on Bone Marrow Histology and Ultrastructure in Immune Induced Aplastic Anemia Mice

Objective To investigate the influence of angelica sinenisis injection on bone marrow histology and ultrastructure in immune-induced aplastic anemia(AA) mice.Methods Female Balb/c mice were divided into normal group,model group,therapy group and contrast group.All mice were killed by cutting neck on the 12 th day, ulnas and femur were taken out. The bone marrow histology section and ultrastructure of mice ulnas were observed. The quantities of hematopoietic cells were counted. The number of femur bone marrow mononucleate cells (MNC) were measured.Results The quantities of hematopoietic cells in model group were lower than those in normal group (P<0.01),but they increased in therapy group(P<0.01). The ultrastructure observation showed that the mitochondria and endoplasmic reticulum of hematopoietic cells in modle group had been reduced and aberrant, the blood sinus cavity were expanded and the wall of sinus were broken. In therapy group, the mitochondria and endoplasmic reticulum were plentiful and normal in shape, and the blood sinus were almost normal. No obvious difference between therpy group and contrast group in MNC counting and hematopoietic microenvironment.Conclusions Angelica sinenisis injection performs a protective role in immune-induced AA mice by increasing the quantity of hematopoietic cells and improving the structure of hematopoietic microenvironment.