| HPLC法测定香砂六君合剂中橙皮苷的含量 |
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| 引用本文： | 焦志峰,迟栋. HPLC法测定香砂六君合剂中橙皮苷的含量[J]. 光明中医, 2011, 26(4): 695-696. DOI: 10.3969/j.issn.1003-8914.2011.04.038 |
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| 作者姓名： | 焦志峰 迟栋 |
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| 作者单位： | 河南南阳医学高等专科学校,南阳,473000 |
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| 摘 要： | 目的建立香砂六君合剂中橙皮苷的HPLC含量测定方法。方法采用SunfireC18（4.6mm×150mm,5μm）,乙腈-0.3%磷酸（22：78）作为流动相,流速为1.0ml/min,紫外检测波长为283,柱温为35℃。结果在9.36～74.88μg/ml范围内,进样量与峰面积积分值呈良好的线性关系,r=0.9998,平均回收率为99.13%,RSD为0.63%（n=6）。结论方法灵敏、简便,结果准确,重现性好,可用于香砂六君合剂的质量控制。
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| 关 键 词： | HPLC 香砂六君合剂 橙皮苷 |
Determination of Orange Popeel Glucoside in Xiangsha liujun Mixture by HPLC |
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| Jiao Zhifeng,Chi Dong. Determination of Orange Popeel Glucoside in Xiangsha liujun Mixture by HPLC[J]. Journal of Traditional Chinese Medicine, 2011, 26(4): 695-696. DOI: 10.3969/j.issn.1003-8914.2011.04.038 |
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| Authors: | Jiao Zhifeng Chi Dong |
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| Affiliation: | Jiao Zhifeng Chi Dong(Nanyang Medicine College,Nanyang 473000) |
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| Abstract: | Objective To establish a HPLC method for the determination of Orange Popeel Glucoside in Xiangsha liujun mixture.Method The chromatographic system consisted of Sunfire C18(4.6 × 150mm,5μm) column,mobile phase was acetonitri-waters with 0.3% phosphoric acid(22:78),The flow rate was 1.0ml /min,the detecting wavelength was 283nm,and the column temperature was 35℃.Results The Orange Popeel Glucoside have a good tincar rolatim in the range of 9.36-74.88μg /ml(r = 0.9998),the average recovery was 99.13% and RSD = 0.63%(n = 6).Conclusion The established method is accuracy,sensitive and simple.It can be used for quality control of Xiangsha liujun mixture. |
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| Keywords: | HPLC |
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