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Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen
Authors:Loskutoff Naida M  Huyser Carin  Singh Raksha  Walker David L  Thornhill Alan R  Morris Lynn  Webber Lynne
Affiliation:a The Bill and Berniece Grewcock Center for Conservation and Research, Omaha, Nebraska
b Department of Obstetrics and Gynaecology, University of Pretoria, Pretoria Academic Hospital, Gauteng, South Africa
c Department of Obstetrics and Gynecology, Mayo Clinic, College of Medicine, Rochester, Minnesota
d National Institute for Communicable Diseases, Johannesburg, Gauteng, South Africa
e Lancet Laboratories, Johannesburg, Gauteng, South Africa
Abstract:
Keywords:Human sperm processing   washing   trypsin   density gradient centrifugation   HIV   HCV
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