急性缺氧暴露对豚鼠耳蜗热休克蛋白90表达的影响

目的:观察热休克蛋白90(heat shock protein 90,HSP 90)在急性缺氧后早期豚鼠耳蜗中的表达变化特点。方法:利用自制缺氧舱使实验动物缺氧,应用免疫组化SABC法结合图象分析技术检测正常和急性缺氧后早期豚鼠耳蜗HSP 90 的表达。结果:HSP 90在正常和缺氧损伤后豚鼠耳蜗各回均有不同程度的染色,主要阳性部位是螺旋神经节、螺旋器、血管纹和螺旋韧带。但缺氧损伤后早期HSP 90在耳蜗各阳性部位的表达均有增强,其中在螺旋神经节和螺旋器的表达增强最明显(P<0．01)。结论:HSP 90在正常和缺氧豚鼠耳蜗组织均有表达,缺氧可明显诱导HSP 90在耳蜗组织的表达增强。HSP 90可能对缺氧损害后耳蜗功能的恢复起作用。

Effects of exposure to acute hypoxia on expression of heat shock protein 90 in cochlea of guinea pig

To investigate the expression of heat shock protein 90(HSP 90) in cochlea of guinea pig after exposure to acute hypoxia. Methods: The animals were put in the hypoxic gas chamber for 1 h and then their cochlea were taken out. The expression of HSP 90 in cochlea of these animals was examined by immunohistochemistry and image analysis system. Results: In both normal group and hypoxic group, the HSP 90 was stained in cochlea, but the expression of HSP 90 was significantly enhanced after hypoxic exposure, especially in spiral ganglion, organ of Corti, stria vascularis and spiral ligament . The enhancement of expression of HSP 90 was more obvious at organ of Corti and stria vascularis in hypoxic group than those in normal group. Conclusion: HSP 90 is expressed in cochlea of normal and hypoxic guinea pigs and hypoxia is of an obvious revulsive effect on the expression of HSP 90 in cochlea.