急性期神经根型颈椎病模型的建立

目的建立快速、准确、简单、经济的急性神经根型颈椎病模型。方法采用椎管插线法,对大鼠颈髓背根神经造成持续卡压,建立神经根型颈椎病动物模型。并对造模后大鼠的一般情况、运动功能、感觉功能、局部病理改变等进行了一系列观察。结果造模后大鼠在1周之内出现了明显的外周神经症状,机械痛阈明显降低,体感诱发电位传导时间延长,背根神经节内神经元坏死、后跟神经脱髓鞘、脊髓后索及外侧索空泡样变等病理改变。但是动物的运动能力、肱二头肌张力、肌肉重量等均没有明显变化。结论利用椎管插线法,可以使大鼠出现明显的感觉神经系统障碍的表现。该法可以作为研究急性期神经根型颈椎病和神经根性疼痛的研究模型。

Establishment of model of acute cervical spondylotic radiculopathy

Objective To establish the model of acute cervical spondylotic radiculopathy in a fast,accurate,easy and simple way.Method To use the intravertebral nylon suture to block continuously rat spinal dorsal root and establish the animal model of cervical spondylotic radiculopathy.The general state,kinetism,sensibility and local pathological changes were observed after the establishment of the model.Result The serious symptoms of peripheral nerves were observed,mechanical pain threshold was decreased,the conduction time of somatosensory evoked potential(SEP) was prolonged,necrosis of neurons in dorsal root ganglion,demyelination of dorsal root and vacuolar degeneration of posterior funiculus and lateral funiculus of spinal cord were observed.But the kinetism,tension of biceps brachii muscle and muscle weight were not changed in model.Conclusion The intravertebral nylon suture can make rats to have significant obstruction of sensory nervous system.The model can be used for the research of acute cervical spondylotic radiculopathy and radiculalgia.