| 利什曼原虫无鞭毛期的低温保存及其存活情况的观察 |
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| 引用本文： | 许永湘,杨玥涛,包意芳. 利什曼原虫无鞭毛期的低温保存及其存活情况的观察[J]. 寄生虫病与感染性疾病, 1993, 0(2) |
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| 作者姓名： | 许永湘 杨玥涛 包意芳 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所，中国预防医学科学院寄生虫病研究所，中国预防医学科学院寄生虫病研究所 上海 2000025，上海 2000025，上海 2000025 |
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| 摘 要： | 本文应用感染杜氏利什曼原虫的病鼠肝、脾组织,保存在液氮内,分别在第3、30和240d以及120和370d复苏后把肝、脾组织块,接种NNN基内,前鞭毛体生长良好,在保存至第105和370d复苏的肝、脾组织块,接种背纹仓鼠腹腔后均获内脏感染。表明液氮保存感染利什曼原虫的动物肝、脾是一种良好的保种方法。
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| 关 键 词： | 杜氏利什曼原虫 无鞭毛期 低温保存 |
OBSERVATION OF LOW TEMPERATURE PRESERVATION AND SURVIVAL OF AMASTIGOTE OF LEISHMANIA PARASITE |
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| Xu Yongxiang,Yang Yuetao,Bao Yifang Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai. OBSERVATION OF LOW TEMPERATURE PRESERVATION AND SURVIVAL OF AMASTIGOTE OF LEISHMANIA PARASITE[J]. Parastoses and Infectious Diseases, 1993, 0(2) |
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| Authors: | Xu Yongxiang Yang Yuetao Bao Yifang Institute of Parasitic Diseases Chinese Academy of Preventive Medicine Shanghai |
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| Affiliation: | Xu Yongxiang,Yang Yuetao,Bao Yifang Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai 200025 |
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| Abstract: | In this experiment, the liver and spleen tissues of rats infected with Leishmania donovani were preserved in liquid nitrogen. The promastigotes cultivated in NNN medium were inoculated into the tissue masses of liver and spleen revived on 3d, 30d, 240d and 120d, 370d respectively and found to grow well. When Cricetulus barabensis was inoculated intraperitoneally with the liv- er and spleen tissues revived on 105d and 370d, visceral infection developed. The results demon- strated that liquid nitrogen preservation of animal liver and spleen infected with Leishmania para- site was a good method for maintenance of Leishmania species. |
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| Keywords: | Leishmania donovani amastigote low temperature presrvation |
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