| HPLC法测定清瘟解毒片中芍药苷的含量 |
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| 引用本文： | 易润青,李艺,宋粉云.HPLC法测定清瘟解毒片中芍药苷的含量[J].广东药学院学报,2009,25(4):379-381. |
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| 作者姓名： | 易润青 李艺 宋粉云 |
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| 作者单位： | 广东药学院药科学院,广东广州,510006 |
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| 基金项目： | 广东省自然科学基金资助项目 |
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| 摘 要： | 目的测定清瘟解毒片中芍药苷的含量。方法采用反相高效液相色谱法，Diamonsil—C18柱，甲醇旬．05mol／LKH2PO4（体积比30：70）为流动相，流速为1．0mL·min^-1，检测波长230nm，柱温为室温。结果芍药苷质量浓度在4．95～59．4μg/mL间具有良好的线性关系（r=0．9997），芍药苷平均回收率为104．1％，方法精密度（RSD）为0．90％（n=6）。结论该法可用于清瘟解毒片中芍药苷的含量测定。关键词：清瘟解毒片；芍药苷；高效液相色谱法；含量测定
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| 关 键 词： | 清瘟解毒片 芍药苷 高效液相色谱法 含量测定 |
Determination of paeoniflorin in Qingwen Jiedu tablets by RP-HPLC |
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| YI Run-qing,LI Yi,SONG Feng-yun.Determination of paeoniflorin in Qingwen Jiedu tablets by RP-HPLC[J].Academic Journal of Guangdong College of Pharmacy,2009,25(4):379-381. |
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| Authors: | YI Run-qing LI Yi SONG Feng-yun |
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| Institution: | YI Run-qing,LI Yi,SONG Feng-yun(School of Pharmaceutical Sciences,Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China) |
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| Abstract: | Objective To determine the content of paeoniflorin in Qingwen Jiedu tablets. Methods RP-HPLC method for the determination of paeoniflorin in Qingwen Jiedu tablets was established. The chromatographic column was Diamonsil-C18. The mobile phase was methanol-0.05 mol/L KH2PO4（30： 70）. The flow rate was 1.0 mL·min^-1, and the detection wavelength was 230 nm. The column temperature was 25°. Results The linear range of Paeoniflorin was 4.95-59.4μg/mL（ r =0.999 7 ）. The average recovery for Paeoniflorin was 104.1%, precision of the method was 0.90% （RSD, n = 6）. Conclusion The method can be used in assay of paeoniflorin of the preparation. |
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| Keywords: | Qingwen Jiedu tablets paeoniflorin RP-HPLC assay |
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