灵芝孢子在降低维甲酸诱导胚胎小鼠发生神经管畸形过程中对Cdk4表达的影响

目的 探讨灵芝孢子在降低维甲酸诱导胚胎小鼠发生神经管畸形过程中对依赖细胞周期蛋白激酶4(CDk4)表达的影响.方法 给予实验对照组和灵芝孢子组妊娠E7.75d的小鼠一次性胃饲维甲酸,造成这2组孕鼠的胚胎出现神经管畸形.然后,给予灵芝孢子组孕鼠胃饲灵芝孢子溶液.在孕期E10.5d时取出2组孕鼠的胚胎,应用免疫荧光组织化学染色和Western blotting检测胚胎神经管神经上皮依赖细胞周期蛋白激酶4(Cdk4)的表达情况.结果 在灵芝孢子组可以观察到形态正常的胚胎中,神经管的神经上皮细胞有Cdk4的表达,其表达水平与空白对照组及正常对照组相比较没有明显差异.在灵芝孢子组形态异常的胚胎中,其神经管的神经上皮细胞也有cdk4的表达,但其表达水平与空白对照组及正常对照组相比较是低的.结论 灵芝孢子可能是通过促进神经管神经上皮细胞上调Cdk4的表达来减轻维甲酸诱导的胚胎小鼠神经管畸形.

THE EFFECT OF GANODERMA SPORES ON THE EXPRESSION OF Cdk4 IN EMBRYONIC NEURAL TUBE DEFFECTS INDUCED BY RETINOIC ACID

Objective To explore the expression of Cdk4 of neural epithelia,when treated with ganoderma spores in embryonic neural tube defects(NTDs) induced by retinoic acid in pregnant mice. Methods When E7.75d,mice of the ganoderma spores and the control groups were given intragastrically onetime retinoic acid.Then the ganoderma spores solution was given intragastrically to the ganoderma spores group.At E10.5d,immunofluorescence histochemistry and Western blotting were applied to examine the expression of Cdk4. Results The expression of Cdk4 was detected in the neural epithelia of the normal embryos in the ganoderma spores group.The abundance of Cdk4 was no difference between the blank control group and the normal control group.Although the expression of Cdk4 was detected in the neural epithelia of the abnormal embryos in the ganoderma spores group,its level was obviously lower than that in the blank control group or the normal control group.Conclusion Ganoderma spores may promote the expression of Cdk4 in the neural epithelia of neural tube to reduce the occurrence of embryonic NTDs induced by retinoic acid in pregnant mice.