多重聚合酶链反应在肺炎链球菌血清学分型中的应用

肺炎链球菌是儿科感染中常见的病原菌之一。基于荚膜多糖抗原的特点,传统荚膜肿胀实验可将肺炎链球菌分为46个血清群,90多种血清型。近年来随着分子生物学技术的发展,多重聚合酶链反应(MPPCR)技术不断成熟并逐步代替常规的荚膜肿胀实验,用于血清学分型。该技术具有简便快速、成本相对较低等优点,能有效的监测临床上肺炎链球菌流行血清型。

Application of multiplex polymerase chain reaction for detecting the serotypes of Streptococcus pneumoniae

Streptococcus pneumoniae is one of common pathogens causing pediatric infections.Owing to the characteristic of capsular polysaccharide,Streptococcus pneumoniae can be differentiated into 40 serogroups and more than 90 serotypes by conventional capsular quellung reaction.Recently,with the development of molecular biological techniques,multiplex polymerase chain reaction （MP-PCR）gradually replaces the conventional capsular quellung reaction,which is used to detect the serotypes of Streptococcus pneumoniae.Furthermore,MP-PCR has the advantages of easy and rapid operation and low costing,which could provide the basis for monitoring the prevalent serotypes of Streptococcus pneumoniae in clinic.