Abstract: | A quantitative method of determination of the activity of multiple forms of superoxide dismutase (SOD), based on separation of the forms of the enzyme of gel electrophoresis, elution from the gel, and determination of inhibition of the reduction of nitro-BT in a system of NADH and phenazine metasulfate, is described. Partially purified bovine erythrocyte SOD was shown to separate into three forms; the activity of form 2 is much lower than the degree of its staining for protein.Institute of Clinical and Experimental Medicine, Academy of Medical Sciences of the USSR, Siberian Branch, Novosibirsk (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 6, pp. 762–764, June, 1977. |