肾小球纤维蛋白沉积对肾脏炎症反应的影响及机制研究

目的探讨调控纤维蛋白沉积水平对肾脏炎症反应的影响及其机制。方法实验分脂多糖诱导大鼠肾小球纤维蛋白沉积（LPS组）；氨甲环酸增加纤维蛋白沉积（TL组）：尿激酶减少纤维蛋白沉积（UT组）。肾组织单核细胞趋化蛋白-1（MCP-1）及血管内皮钙黏附蛋白（VE—Cad）mRNA和蛋白质表达分别用RT—PCR和免疫组织化学检测。结果（1）TL组与LPS．UT组纤维蛋白沉积、炎细胞数目差异有统计学意义（P〈0．05）；（2）TL组与LPS，UT组的MCP-1、VE—Cad mRNA及蛋白质表达水平差异亦有统计学意义（P〈0．05）。结论肾小球纤维蛋白沉积具有促炎症作用；纤维蛋白沉积的促炎症作用与其调节MCP-1、VE-Cad表达有关。

Effect of glomerular fibrin deposition on inflammatory response and mechanism in rats

Objective To investigate the proinflammatory effects of glomerular fibrin deposition on inflammatory response and its mechanisms in rats. Methods Female Wistar rats were randomly divided into four groups (n=8 per group): normal control group (NC group) injected with saline alone; Lipopolysaccharide (LPS) group with fibrin deposition induced with LPS; TL group treated with LPS and tranexamic acid;UT group treated with LPS, tranexamic acid and urokinase. Tranexamic acid and urokinase were used to increase and decrease fibrin deposition, respectively. Fibrin and macrophages were detected by immunofluorescence and immunohistochemical staining. Monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial-cadherin (VE-Cad) protein and mRNA expression were analyzed by immunohistochemistry and RT-PCR, respectively. Results Fibrin deposition and macrophages of TL group were significantly higher than those of LPS, UT groups (P < 0.05). And there were significant differences between TL and LPS, UT in renal tissue MCP-1 , VE-Cad mRNA and protein expression (P < 0.05). Conclusions Fibrin deposition contributes to renal inflammatory response. Proinflammatory effects of fibrin deposition may be involved in the control of MCP-1, VE-Cad expression.