氟中毒氧化应激与细胞凋亡关系的研究

目的 研究氟、硒对大鼠肝细胞氧化应激和细胞凋亡的影响.方法 大鼠经饮水加氟化钠(150mg/L)或/和亚硒酸钠(2mg/L)10周后,检测肝细胞凋亡百分率、细胞周期构成比、活性氧(ROS)和还原型谷胱甘肽(GSH)含量以及脂质过氧化物(LPO)水平.结果 氟可使肝细胞凋亡百分率明显升高,S期细胞数增多,ROS和LPO水平升高,GSH含量下降;硒通过稳定细胞GSH含量可拮抗氟的毒性作用,使组织中ROS、LPO水平下降,GSH含量升高,凋亡百分率下降.结论 硒可部分拮抗氟化物诱导的肝细胞脂质过氧化和凋亡,氧化应激参与了介导细胞凋亡的过程.

Relationship Study Between Oxidative Stress and Apoptosis in Fluorosis Rats

Objective To study effects of selenium and fluoride on oxidative stress and apoptosis in rat liver tissue.Methods The apoptosis,cell cycle,reactive oxygen species(ROS),reduced glutathione(GSH)content and lipid peroxidation (LPO)level in rat liver tissue were measured in the presence or absence of seleium(2mg/L)and fluoride(150mg/L).Results Fluoride enhanced apoptosis in rathepatocy tes,increased the number of cells in Sphase,increased ROS and LPO levels.Fluoride decreased the cellular GSH content.Selenium could antagonize the fluor ide-induced cytotoxicity by stabilizing the cellular GSH content,and by reducing ROS and LPO levels,respectively.Areduction of apoptosis in rathepatocytes after selenium treatment was observed.Conclusion Selenium can partially antagonism the fluo ride-induced lipid per oxidation and apoptosis in rathepatocytes.ROS participated the process of apoptosis.