溃疡性结肠炎中医分型与T细胞亚群关系

目的:探讨溃疡性结肠炎(ulcerative colitis,UC)中医分型与结肠黏膜T细胞亚群CD3+、CD4+、CD8+的关系。方法:将UC病人按中医分型分为4组,并设对照组。所有患者用电子结肠镜取结肠黏膜组织3~5块,应用免疫组化法检测T细胞亚群CD3+、CD4+、CD8+。结果:UC患者结肠黏膜T淋巴细胞亚群的变化以CD8+细胞水平升高及CD4+/CD8+比值下降为主,湿热内蕴型CD4+细胞明显下降,CD8+细胞明显升高;气滞血瘀型CD4+细胞明显下降,CD8+细胞轻度升高;脾肾两虚型CD4+细胞轻度下降,CD8+细胞轻度升高;阴血亏虚型CD4+细胞轻度下降,CD8+细胞明显升高。结论:UC的中医分型与T细胞亚群关系密切,检测UC患者结肠黏膜中T细胞亚群可为中医对UC的分型提供理论依据。

Study on the Relationship between Chinese Medicine Grouping of Ulcerative Colitis and T Cell Subgroup

Objective:To investigate the relationship between UC practitioner of Chinese medicine grouping and T cell subgroup(CD3+,CD4+,CD8+) in intestinal mucosa.Methods:UC patients were divided into four group by practitioner of Chinese medicine Grouping,each type is a suite,another set up control group,there are 8～15 people in each suite,each patient were recipe 3～5 pieces intestinal mucosa tissue through electronic colonoscope,T cell subgroup(CD3+,CD4+,CD8+) were detected through immunohistochemistry after completing exemplars collection.Results:The change of UC patients,T cell subgroup in intestinal mucosa is overall that CD8+cellular level is to heighten and the ratio of CD4+/CD8+is to lower.The CD4+ cellular level is to lower obviously and the CD8+cellular level is to heighten obviously in the endoretention of damp heat;The CD4+ cellular level is to lower obviously and the CD8+cellular level is to heighten lightly in pneuma stagnant blood stasis;The CD4+cellular level is to lower lightly and the CD8+cellular level is to heighten lightly in asthenia of both the spleen and kidney;The CD4+cellular level is to lower lightly and the CD8+cellular level is to heighten obviously.Conclusion:The relation between UC practitioner of Chinese medicine grouping and T cell subgroup(CD3+,CD4+,CD8+) in intestinal mucosa is intimate.The detection of T cell subgroup of UC patients in intestinal mucosa may provid theory accordance with for UC practitioner of Chinese medicine grouping.