吡格列酮对2型糖尿病大鼠胃促生长素表达的影响

目的探讨吡格列酮对2型糖尿病大鼠血浆胃促生长素水平及其胃壁组织mRNA表达水平的影响。方法SPF级近交系雄性健康Wistar大鼠，以高脂饲料联合小剂量链脲佐菌素（30mg/kg）建立2型糖尿病模型，分为3组：正常对照组（NC组，10只）、糖尿病吡格列酮治疗组（DM-PIO组，12只）和糖尿病未治疗组（DM组，12只）。吡格列酮剂量为20mg·kg^-1·d^-1。用酶免法测定血浆胃促生长素水平，以RT-PCR检测胃壁组织mRNA表达水平。结果DM组和DM-PIO组血浆胃促生长素及其胃壁组织mRNA表达水平显著低于NC组（P均＜0.05），DM-PIO组与DM组相比有上升趋势，但差异无统计学意义（P＞0.05）。血浆胃促生长素水平与体重（P＜0.05）、HOMA-IR(P＜0.01)和瘦素水平(P＜0.05)呈负相关。结论2型糖尿病大鼠血浆胃促生长素及其在胃壁组织的mRNA表达水平均显著降低，未发现吡格列酮对其有显著影响。

Effects of pioglitazone on the ghrelin expression in type 2 diabetic rats

To explore the effects of pioglitazone on the plasma ghrelin levels and the ghrelin mRNA expression in the stomach of type 2 diabetic rats. MethodsWistar rats were fed with a high fat diet for 8 weeks and then treated with a low dose of STZ(30mg/kg) by intra peritoneal injection to establish type 2 diabetic rat models, then they were divided into three groups: the control group(NC, n=10), the diabetes group(DM, n=12) and the pioglitazone treatment group(DM-PIO, n=12). Rats in the DM-PIO group were treated with pioglitazone(20mg·kg^-1·d^-1) by intra-gastric administration. All the animals were sacrificed 8 weeks later. The blood glucose, lipids, insulin, leptin and ghrelin were determined, and the ghrelin mRNA expression in the stomach was determined by RT-PCR. ResultsThe DM and DM-PIO groups had significant reduced plasma levels of ghrelin and mRNA expression levels in the stomach compared with the NC rats (P＜0.05 for each group). Plasma ghrelin and mRNA expression levels were higher in the DM-PIO rats than in the DM rats, which was not significant (P＞0.05). By the correlation analysis, plasma ghrelin levels were negatively correlated with body weight (P＜0.05), HOMA IR (P＜0.01) and leptin P＜0.05) in diabetic rats. ConclusionThe plasma ghrelin levels and the expression of ghrelin in diabetic rats are significantly reduced. Pioglitazone can not change the plasma levels of ghrelin and its expression in the stomach.