| 重构型人caspase-8基因原核表达载体的构建及其表达 |
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| 引用本文： | 桂俊豪,金明,赵晶,贾林涛,许彦鸣,于翠娟,王成济,杨安钢,周常文,仇东辉. 重构型人caspase-8基因原核表达载体的构建及其表达[J]. 西北国防医学杂志, 2003, 24(5): 364-366 |
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| 作者姓名： | 桂俊豪 金明 赵晶 贾林涛 许彦鸣 于翠娟 王成济 杨安钢 周常文 仇东辉 |
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| 作者单位： | 1. 兰州军区乌鲁木齐总医院临床医学研究所,新疆,乌鲁木齐,830000
2. 第四军医大学生物化学与分子生物学教研室,陕西,西安,710033 |
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| 摘 要： | 目的：构建三种重构型人caspase—8基因的原核表达载体，转染大肠杆菌并诱导其表达。方法：将人caspase—8催化结构域基因及两种大、小亚基基因次序颠倒的重构型人caspase—8基因克隆人原核表达载体pBV220，转染大肠杆菌并诱导表达。结果：成功构建了三种重构型人caspase—8基因的原核表达载体。转染大肠杆菌后，经温度诱导，重构型人caspase—8基因获得了高效的表达。结论：在大肠杆菌中成功表达了三种重构型人caspase—8基因。
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| 关 键 词： | 人caspase—8基因 pBV220 原核表达 |
| 文章编号： | 1007-8622(2003)05-0364-03 |
| 修稿时间： | 2003-05-15 |
Construction and expression of prokaryotic expression vectors carrying recombinant human caspase-8 genes |
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| GUI Jun-hao ,JIN Ming ,ZHAO Jing ,et al.. Construction and expression of prokaryotic expression vectors carrying recombinant human caspase-8 genes[J]. Medical Journal of National Defending Forces in Northwest China, 2003, 24(5): 364-366 |
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| Authors: | GUI Jun-hao JIN Ming ZHAO Jing et al. |
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| Affiliation: | GUI Jun-hao 1,JIN Ming 2,ZHAO Jing 2,et al. |
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| Abstract: | Objective:To construct three recombinant human caspase-8 genes into prokaryotic expression vectors and express them in E.coli.Methods:Subcloned the catalytic domain gene of human caspase-8 and two reconstructed human caspase-8 genes in which small subunits were settled prior to large subunits into prokaryotic expression vector pBV220 and induced their expression in E.coli.Results:Three prokaryotic expression vectors carrying recombinant human caspase-8 genes were constructed successfully and expressed efficiently in E.coli.Conclusion:Three recombinant human caspase-8 genes were expressed in E.coli successfully. |
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| Keywords: | Recombinant Human caspase-8 gene pBV220 Prokaryotic expression |
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