TAM对C6细胞生长与侵袭能力的影响

目的:通过观察TAM对体外培养C6细胞生长状况、迁移能力与MMP-2蛋白表达的影响,为TAM用于胶质瘤的治疗提供理论依据。方法:观察不同剂量TAM对大鼠C6胶质瘤细胞生长曲线、细胞倍增时间及细胞形态的影响,研究TAM对C6细胞的生长抑制作用;细胞划痕法检测不同剂量TAM对C6细胞迁移能力的影响;FITC标记的可激活穿膜肽检测不同剂量TAM对C6细胞MMP-2蛋白表达的影响。结果:TAM显著抑制C6胶质瘤细胞的生长,且存在剂量、时间依赖关系;TAM显著抑制C6细胞迁移,存在剂量依赖关系;TAM对C6细胞活性MMP-2蛋白的表达未见明显影响。结论:虽然TAM不能抑制C6细胞MMP-2蛋白的表达,但可显著抑制其生长与迁移,因此有望用于胶质瘤的化学治疗。

TAM suppresses C6 glioma cells growth and migration in vitro

Objective:To investigate the growth,migration,and the expression of MMP-2 of C6 glioma cells treated with tamoxifen.Methods:Culture curve,cells doubling time,and morphological changes of C6 glioma cells,treated with various concentration of TAM,were investigated to study the correlation between C6 glioma cells growth and TAM.Cell scratching test was used to investigate the correlation between TAM and C6 cells migration.ACPP-FITC was used to detect the expression of active MMP-2 of C6 glioma cells treated with various concentration of TAM.Results:TAM significantly suppressed the growth of C6 glioma cells in a time and dose-dependent manner.TAM suppressed the migration of C6 glioma cells conspicuously.TAM didn't affect the expression of MMP-2 of C6 glioma cells.Conclusion:TAM can significantly suppress the growth and migration of C6 glioma cells,although can' t affect the expression of active MMP-2.Therefore,TAM can be used as a chemotherapy agent for gliomas.