| 靶向HER2的抑制胃癌细胞功能性单抗的制备和鉴定 |
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| 引用本文： | 孙立新,冉宇靓,胡海,遇珑,周转,赵西路,杨治华. 靶向HER2的抑制胃癌细胞功能性单抗的制备和鉴定[J]. 中国肿瘤生物治疗杂志, 2008, 15(1): 14-19. DOI: 10.3872/j.issn.1007-385X.2008.01.004 |
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| 作者姓名： | 孙立新 冉宇靓 胡海 遇珑 周转 赵西路 杨治华 |
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| 作者单位： | 中国医学科学院,中国协和医科大学肿瘤研究所,细胞及分子生物学研究室,北京,100021 |
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| 基金项目： | 国家自然科学基金 , 国家高技术研究发展计划(863计划) , 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的: 制备可特异识别人表皮生长因子受体2(human epidermal growth factor receptor2,HER2)膜抗原的单克隆抗体，筛选出亲和力较高并对胃癌细胞有明显抑制作用的克隆。方法：以高表达HER2膜抗原的人胃癌细胞系NCIN87免疫BABL/c小鼠，免疫小鼠脾细胞与小鼠骨髓瘤细胞SP2
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| 关 键 词： | 胃癌 NCI-N87细胞系 人表皮生长因子受体-2 单克隆抗体 靶向 胃癌细胞增殖 功能性 单抗 制备和鉴定 cells gastric carcinoma monoclonal antibodies 应用 治疗 改造 细胞生长 高通量 线形结构 电泳 石蜡包埋 别部 抑制率 细胞的增殖 人胃癌 |
| 文章编号： | 1007-385X（2008）01-0014-06 |
| 收稿时间： | 2007-11-30 |
| 修稿时间： | 2008-01-05 |
Preperation and identification of HER2-targeting monoclonal antibodies suppressing gastric carcinoma cells |
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| SUN Li-xin,RAN Yu-liang,HU Hai,YU Long,ZHOU Zhuan,ZHAO Xi-lu and YANG Zhi-hua. Preperation and identification of HER2-targeting monoclonal antibodies suppressing gastric carcinoma cells[J]. Chinses Journal of Cancer Biotherapy, 2008, 15(1): 14-19. DOI: 10.3872/j.issn.1007-385X.2008.01.004 |
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| Authors: | SUN Li-xin RAN Yu-liang HU Hai YU Long ZHOU Zhuan ZHAO Xi-lu YANG Zhi-hua |
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| Affiliation: | Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Department of Cellular Biology and Molecular Biology,Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China |
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| Abstract: | |
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| Keywords: | gastric carcinoma NCI-N87 cell line human epidermal growth factor receptor-2 monoelonal antibody |
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