GSTM1基因多态及膳食因素与肺癌关系的研究

目的 研究谷胱苷肽硫转移酶M1(GSTMl)基因多态性及膳食因素与广州地区肺癌发生的关系。方法 采用成组病例-对照研究方法，病例58例，对照62例，制定统一的调查表进行调查，用PCR检测GSTM1基因多态性。结果 GSTM1基因多态性与肺癌危险性关系无显著性差异(OR1．73，95％CI0．84～3．58)；Logistic单因素分析显示：胡萝卜摄入频率与肺癌发生的危险性呈负相关(OR0．24，95％CI0．10～0．58)，而用动物油脂炒菜与肺癌的发生呈正相关(OR5．34，95％CI1．13～20．16)。非条件logistic多因素回归分析校正吸烟等非膳食烹调因素后，胡萝卜摄入频率(OR0．18，95％C10．05～0．65)仍与肺癌发生负相关；不常吃胡萝卜联合GSTM1缺失发生肺癌的危险性显著增加(OR6．30，95％CI1．88～21．05)。结论 GSTM1基因单独作用时与肺癌关系不明显；用动物油脂炒菜显著增加肺癌发生的危险性。常摄入胡萝卜可显著降低肺癌发生危险性。GSTM1基因多态性与胡萝卜摄入间存在协同作用。

Study on relationship between GSTM1 polymorphism,diet factors and lung cancer

Objective To study the relationship of polymorphism of GSTM1 and diet factors with lung cancer risk in Guangzhou.Methods A case-control study which included 58 cases of lung cancer and 62 controls was conducted.All subjects were investigated with a uniform questionaire.The genotypes of GSTM1 were detected by PCR.Results There was no significant association between the genotypes of GSTM1 and the risk of lung cancer ( OR 1 73,95% CI 0 84-3 58);The consumption of carrot was significant inversely correlated with the risk of lung cancer ( OR 0 24,95% CI 0 10-0 58).Cooking with animal oil was negatively and significantly associated with the risk of lung cancer ( OR 5 34,95% CI 1 13-20 16).There was significant association between lung cancer and the consumption of carrot ( OR 3 53,95% CI 1 24-13 03) after multivariable-adjusted logistic regression model.Conclusion There was not significant association between the polymorphism of GSTM1 and the risk of lung cancer.Cooking with animal oil significantly increased the risk of lung cancer.Consumption of carrot significantly reduced the risk of lung cancer.A combined effect of polymorphism of GSTM1 and consumption of carrot on the risk of lung cancer was suggested.