子宫内膜异位症腹水内血管内皮细胞生长因子及白介素8的测定

目的 :探讨子宫内膜异位症 (内异症 )患者腹腔液中血管内皮细胞生长因子 (VEGF)及白细胞介素 8(IL - 8)在内异症发病中的作用。方法 :采用双抗体夹心酶联免疫吸附法 (ELISA)检测 2 5例内异症患者 (Ⅰ、Ⅱ期 13例 ,Ⅲ、Ⅳ期 12例 ) ,15例非内异症患者 (对照 )腹腔液中VEGF及IL - 8的含量 ,分析VEGF、IL - 8的浓度及其与内异症分期 [美国生殖协会修正标准分期 (R -AFS) ]的相关性。结果 :内异症患者腹腔液中VEGF及IL - 8的浓度明显高于对照组 ( P <0 0 5及 P <0 0 1) ,VEGF及IL - 8与R -AFS分期无相关 ,VEGF与IL - 8之间无明显相关。结论 :内异症患者腹水中VEGF、IL - 8的水平升高是腹腔微环境改变导致内异症的可能及部分原因

MEASUREMENT OF VASCULAR ENDOTHLIAL GROWTH FACTOR AND INTERLEUKIN-8 LEVELS IN PERITONEAL FLUID OF PATIENTS WITH ENDOMETRIOSIS

Objective:To investigate the role of vascular endothelial growth factor(VEGF) and interleukin-8(IL-8) in peritoneal fluid on pathogenesis of endometriosis(EMT).Methods:VEGF and IL-8 contents in peritoneal fluid of 25 cases with EMT (study group) were detected by enzyme linked immunoabsorbent assay(ELISA) and compared with the counterparts of 15 cases without EMT(control group).The correlation analyses between VEGF and IL-8 concentration in peritoneal fluid of EMT patients were performed and compared with the severity of R-AFS score.Result:The peritoneal fluid form patients with endometriosis contained significantly greater amounts of VEGF (194±45pg/ml) and IL-8(1 9±0 53ng/ml) than controls(160±50pg/ml and 1 3±0 4ng/ml,respectively,P<0 05;P<0 01).There was no correlation between R-AFS scores and VEGF or IL-8 levels. There was no correlation between levels of VEGF and IL-8 in peritoneal fluid.Conclusion:Unusual levels of VEGF and IL-8 in peritoneal fluid of EMT patients may be the possible or part reason to change the microenvironment of the peritoneal cavity.