胰岛素抵抗在大鼠实验性非乙醇性脂肪肝中的作用

目的：探讨胰岛素抵抗在非乙醇性脂肪肝（NAFLD）中的作用.方法： 8只SD大鼠随机分成正常对照组（6只）和高脂饮食组（42只）,正常对照组饲养8周,高脂饮食组分为7组,每隔2周处死1组,测定大鼠的体重,肝脏的湿重,空腹真胰岛素（FIns）、血糖（FBG）、血脂水平,观察肝组织学改变.结果： 模型组大鼠喂养8周时出现NAFLD.与正常对照组相比,模型组大鼠的FBG,FIns,TG,高密度脂蛋白胆固醇（HDL-C）,胰岛素敏感指数（ISI）,肝细胞脂肪变程度与对照组相比均有差异.TG, FIns与肝细胞脂肪变程度成正相关,ISI,HDL-C与肝细胞脂肪变程度成负相关.结论： 模型组大鼠存在胰岛素抵抗; 胰岛素抵抗在非乙醇性脂肪肝的发生中起重要作用.

Insulin Resistance in the Pathogenesis of Non-alcoholic Fatty Liver Disease

Objective: To explore the effect of insulin resistance on the pathogenesis of non-alcoholic fatty liver disease(NAFLD).Methods: Forty eight male SD rats were randomly allocated into control group(n=6) fed with standard diet for 8 weeks and model group(n=42) induced by high fat diet.The model group was divided into 7 groups,these rats were sacrificed at the 2,4,6,8,10,12,14wk of the experiment respectively.Body weight,liver weight,fasting blood glucose(FBG),serum triglyceride(TG),fasting serum insulin(FIns) level and liver histology were detected.Results:The livers presented the pathology of hepatocyte steatosis at 8 week in rats of model group.In comparison with the control group,there was difference in FBG,FIns,TG,insulin sensitivity index(ISI),HDL-C and the degree of fatty liver in model group.TG and FIns were positive correlation with the degree of hepatocyte steatosis.Conclusion:Insulin resistance generally presents in rats with NAFLD.Insulin resistance may play an important role in the pathogenesis of NAFLD.