He-Ne激光和弱CO2激光照射加速骨折愈合的比较

背景:弱激光的生物刺激作用已被许多实验研究及临床应用证实,其作用表现在促进组织修复、镇痛、抗炎等方面。目的:观察He-Ne激光和小剂量CO2激光照射对骨折愈合的作用。设计:随机对照动物实验。单位:承德医学院激光医学研究室。材料:选择健康雄性新西兰白兔48只,体质量2.0～2.5kg。方法:实验于1998/2003在承德医学院激光医学研究室完成。①将48只新西兰白兔左桡骨造成实验性骨折,将之分为3组,每组16只,其中一组为对照组,另外两组分别接受28mW/cm2的He-Ne激光照射(He-Ne组)及150mW/cm2的CO2激光照射(CO2组),1次/d,10min/次,直接照射骨折部位。②骨折后15d和30d拍摄X射线片后分批处死动物。取出实验骨,对15d的实验骨,测定骨痂中的钙与胶原含量。对35d的实验骨,测定生物力学抗扭性能。主要观察指标:①各组X射线片观察结果。②各组骨痂中胶原与钙的含量。③各组生物力学抗扭强度测试结果。结果:45只进入结果分析。①骨折后15d,CO2组及He-Ne组的X射线片、骨痂中的胶原及钙含量均优于对照组,且CO2组骨痂中的胶原及钙含量高于He-Ne组犤(341.9±30.1)比(302.1±28.7)mg/g,(197.1±19.7)比(156.5±17.6)mg/g,P<0.05犦。②骨折后35d,CO2激光及He-Ne激光照射组的生物力学抗扭性能均优于对照组,两个照射组之间的差异无显著性。③两个时间得到的X射线片的结果显示CO2激光及He-Ne激光照射组均优于对照组,两个照射组之间的差异无显著性。结论:CO2激光及He-Ne激光照射均促进了骨折愈合,且在骨折后15d时,CO2激光照射的作用效果显著优于He-Ne激光照射,而在骨折后35d时,从临床意义上讲,其差异无显著性。

Effect of He-Ne laser verus low-level CO2 laser irradiation on accelerating fracture healing

BACKGROUND: The effects of low-level laser biostimulation have been proved by various experimental studies and clinical application, which are manifested as tissue repairing, analgesia, antiinflammation, etc.OBJECTIVE: To observe the effects of He-Ne laser and low-level CO2 laser irradiation on fracture healing.DESIGN: A randomized controlled animal experiment. SETTING: Research Room of Laser Medicine, Chengde Medical College.MATERIALS: Forty-eight healthy male New Zealand rabbits, weighing 2.0-2.5 kg, were used.METHODS: The experiments were carried out in the Research Room of Laser Medicine, Chengde Medical College from 1998 to 2003. ①The 48 rabbits were induced into models of experimental fracture of the left radius, and then they were divided into three groups with 16 rabbits in each group: control group, He-Ne laser irradiation group and CO2 laser irradiation group. The fracture areas of the animals in the He-Ne and CO2 laser irradiation groups were respectively irradiated with 28 mW/cm2 He-Ne laser and 150 mW/cm2 CO2 laser for 10 minutes, once a day. ② The animals were killed on the 15th and 35th days after taking roentgenogram respectively. At 15 days, the collagen and calcium contents in callus were detected. At 35 days, the biomechanic anti-torsion strength was examined.MAIN OUTCOME MEASURES: ①Results of roentgenogram in each group; ② Collagen and calcium contents in callus; ③ Results of biomechanic anti-torsion strength.RESULTS: Totally 45 rabbits were involved in the analysis of results. ① On the 15th and day after fracture, the roentgenogram, collagen and calcium contents in callus in the He-Ne and CO2 laser irradiation groups were better than those in the control group, and the collagen and calcium contents in callus were higher in the CO2 laser irradiation group than in the He-Ne laser irradiation group [(341.9±30.1) vs (302.1±28.7) mg/g; (197.1±19.7)vs (156.5±17.6) mg/g, P＜ 0.05]. ②On the 35th days after fracture, the biomechanic anti-torsion functions in the He-Ne and CO2 laser irradiation groups were superior to that in the control group, and there were no significant difference between the two laser irradiation groups. ③ The results of roentgenogram in the He-Ne and CO2 laser irradiation groups were better than that in the control group both on the 15th and 35th days after fracture, and there was no significant difference between the two irradiation groups.CONCLUSION: Both CO2 laser and He-Ne laser irradiations can accelerate the process of fracture healing, and the curative effect of CO2 laser is better than that of He-Ne laser on the 15th day after fracture, but there is no significant difference clinically between the CO2 laser and the He-Ne laser irradiations on the 35th day after fracture.