Nannizzia incurvata in Hue city - Viet Nam: Molecular identification and antifungal susceptibility testing |
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| Authors: | Thi Minh Chau Ngo Phuong Anh Ton Nu Chi Cao Le Minh Tiep Vo Thi Ngoc Thuy Ha Thi Bich Thao Do Phuoc Vinh Nguyen Giang Tran Thi Antonella Santona |
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| Affiliation: | 1. Department of Parasitology, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue 49000, Viet Nam;2. Department of Biomedical Sciences, University of Sassari, Viale S. Pietro 43/b, Sassari 07100, Italy;1. Division of Infectious Diseases, Department of Internal Medicine, Thammasat University, Pathum Thani Province, Thailand;2. Chulabhorn International College of Medicine, Thammasat University, Pathum Thani Province, Thailand;3. Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Thailand;4. American Regent, Norristown, PA, USA;1. Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, D-41065 Mönchengladbach, Germany;2. Institute of Virology and Microbiology, University of Witten/Herdecke, D-58448 Witten, Germany;3. GEN-IAL GmbH, D-53842 Troisdorf, Germany;4. Center for Advanced Microstructures and Devices (CAMD), Louisiana State University, Baton Rouge, LA 70806, United States;1. Department of Microbiology, Tata Medical Center, Kolkata, India;2. Department of Pediatric Oncology, Tata Medical Center, Kolkata, India;1. Laboratório de Radioisótopos, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Campus Pampulha, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;2. Laboratório de Farmacologia da Dor e Inflamação, Universidade Federal de São João del–Rei (UFSJ) - Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil;3. Fundação Ezequiel Dias (FUNED), Belo Horizonte, Minas Gerais, Brazil;4. Laboratório de Farmacognosia, Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Campus Pampulha, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;1. Microbiology Department, Parasitology-Mycology Unit, Faculty of Medicine, Paris University, AP–HP, European Georges-Pompidou Hospital, 75015 Paris, France;2. Microbiology Department, Parasitology-Mycology Unit, Faculty of Medicine, Paris University, Necker-Enfants Maladies Hospital, 75015 Paris, France;3. Medical Intensive Care Unit, Faculty of Medicine, Paris-Descartes University, AP–HP, European Georges-Pompidou Hospital, 75015 Paris, France;4. Fungal Biology and Pathogenicity. Institut Pasteur, Paris, France;5. Dynamyc EA 7380, Paris-Créteil University, Créteil, France;1. Department of Pediatric Infectious Diseases, Dr. Behçet Uz Children''s Diseases and Surgery Training and Research Hospital, ?zmir, Turkey;2. Division of Pediatric Intensive Care Unit, Dr. Behcet Uz Children''s Disease and Pediatric Surgery Training and Research Hospital, Izmir, Turkey;3. Infection Control Committee, Dr. Behçet Uz Children''s Disease and Pediatric Surgery Training and Research Hospital, Izmir, Turkey;4. Department of Microbiology and Clinical Microbiology, Dr. Behçet Uz Children''s Diseases and Surgery Training and Research Hospital, ?zmir, Turkey |
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| Abstract: | BackgroundNannizzia incurvata, a species belonging to the Nannizzia gypsea complex, is considered a neglected pathogen.ObjectiveTo detected N. incurvata isolates from dermatophytosis patients in Hue city - Viet Nam, and test the antifungal susceptibility of this species. Moreover, fungal capability to produce hydrolytic enzymes was evaluated.MethodsPatients’ samples were collected and cultured on Sabouraud-chloramphenicol-cycloheximide medium. Dermatophytes isolates were initially macroscopically and microscopically identified. ITS PCR-RFLP and ITS rDNA sequences were performed to determine and confirm species. An ITS Neighbor-Joining phylogenetic tree evaluated the genetic relationship among isolates. Fungal hydrolytic enzymes were examined, including lipase, phospholipase and protease. Antifungal susceptibility testing was carried out by the disk diffusion method. MICs of itraconazole, voriconazole, and terbinafine against these isolates were determined by the broth microdilution method.ResultsTwelve isolates of N. gypsea complex were preliminary morphologically identified. PCR-RFLP and ITS-rDNA sequencing identified and confirmed dermatophytes as N. incurvata strains, respectively. An evident polymorphism among isolates was highlighted in the phylogenetic tree. All isolates showed the activity of lipase, phospholipase, and protease production. Overall, all N. incurvata isolates were susceptible to itraconazole, voriconazole, clotrimazole, miconazole, and terbinafine. Few isolates were susceptible to griseofulvin, and none of them were susceptible to fluconazole.ConclusionsThere was a presence of polyclonal N. incurvata isolates in dermatophytosis patients from Hue city, identified by PCR-RLFP and confirmed by ITS sequencing. We confirmed PCR-RLFP as a reliable technique to identify this species. Azole and terbinafine are the optimal choices for N. incurvata treatment except for fluconazole. |
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