| 补体1q/肿瘤坏死因子相关蛋白9抑制心肌成纤维细胞的激活和迁移 |
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| 引用本文： | 李香敏,谭延振,张冰,赵荣,金振晓,易定华,孙阳,易蔚,张平.补体1q/肿瘤坏死因子相关蛋白9抑制心肌成纤维细胞的激活和迁移[J].中国体外循环杂志,2022(1):38-43. |
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| 作者姓名： | 李香敏 谭延振 张冰 赵荣 金振晓 易定华 孙阳 易蔚 张平 |
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| 作者单位： | 空军军医大学第二附属医院肾脏内科;空军军医大学第一附属医院心血管外科;空军军医大学第一附属医院老年病科 |
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| 基金项目： | 国家自然科学基金(81770345,51837011);陕西省重点研发计划(2019ZDLSF01-01-02,2020SF-250)。 |
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| 摘 要： | 目的 探究补体1q/肿瘤坏死因子相关蛋白9(CTRP9)对抑制心肌成纤维细胞(CFs)活性的作用及其分子机制.方法 应用MTS法检测SD仔鼠左心室CFs增殖活性,应用免疫荧光染色及Western Blotting检测α-SMA表达评估CFs向肌成纤维细胞转化,应用细胞划痕实验及Transwell实验检测CFs迁徙能力,...
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| 关 键 词： | 补体1q/肿瘤坏死因子相关蛋白9 心肌成纤维细胞 转化生长因子β1 蛋白激酶A |
CTRP9 inhibits the activation and migration of cardiac fibroblasts |
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| Li Xiangmin,Tan Yanzhen,Zhang Bing,Zhao Rong,Jin Zhenxiao,Yi Dinghua,Sun Yang,Yi Wei,Zhang Ping.CTRP9 inhibits the activation and migration of cardiac fibroblasts[J].Chinese Journal of Extracorporeal Circulation,2022(1):38-43. |
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| Authors: | Li Xiangmin Tan Yanzhen Zhang Bing Zhao Rong Jin Zhenxiao Yi Dinghua Sun Yang Yi Wei Zhang Ping |
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| Institution: | (Department of Cardiovascular Surgery,the First Affiliated Hospital,Air Force Medical University,Shaanxi Xi'an 710032,China) |
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| Abstract: | Objective To investigate the effect and molecular mechanism of CTRP9 on reducing the activation and migration of cardiac fibroblasts(CFs). Methods The cell proliferation activity of CFs were detected by MTS kit. The expression of α-SMA was detected by immunofluorescence staining and western blotting to evaluate CFs-to-myofibroblast transformation. Wound healing assay and transwell test were used to test the migration ability of CFs. Western blotting was used to detect collagenⅠ, Ⅲ and CTGF. Results CTRP9 significantly inhibited the increase of TGF-β1-induced CFs proliferation(P<0.001), transformation to myofibroblasts(P<0.01), but enhanced migration ability(P<0.001), and the expression of collagen I, collagen III, and CTGF(P<0.001). PKA inhibitor H-89 blocked those inhibitions of CTRP9(P<0.001, P≤0.05, P<0.001, P<0.001). Conclusion CTRP9 reduces TGF-β1-induced proliferation, transformation to myofibroblasts, migration and the expression of fibrosis-related proteins of cardiac fibroblasts via PKA to exert anti-fibrosis effects. |
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| Keywords: | Complement 1q/tumor necrosis factor related protein 9 Cardiac fibroblasts Transforming growth factor beta 1 Protein kinase A |
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