SOX7在肾细胞癌中的表达及其对肾癌细胞生物学行为的影响

目的:研究Y性别决定基因7(sex determining region Y-box7,SOX7)在肾细胞癌的表达以及对肾癌细胞生物学行为的影响.方法:qRT-PCR和甲基化特异性PCR(MSP)检测SOX7在肾细胞癌组织和肾癌细胞(A498、ACHN、786-O)中的mRNA表达及甲基化状态;免疫组织化学法(immu...

Expression of SOX7 in renal cell carcinoma and its effect on the biological behavior of renal cancer cells

Objective: To investigate the expression of sex determining region Y-box 7(SOX7) in renal cell carcinoma and the effect on the biological behavior of renal cancer cells. Methods:  qRT-PCR and methylation specific PCR (MSP) were used to detect the expression and methylation status of SOX7 in renal cell carcinoma tissues and renal cancer cells (A498, ACHN, 786 O); immunohistochemistry (IHC) was used to detect the protein expression of SOX7 in renal cell carcinoma tissues. The SOX7 mRNA expression in renal cancer cells after 5-Aza-dC/TSA intervention was also detected by qRT-PCR. After transfection of SOX7 overexpression plasmid in renal cancer cells ACHN, cell proliferation, migration and invasion were detected by MTS, clone formation, scratch assay and Transwell assay, respectively. Results:  The qRT-PCR and IHC results showed that the expression of SOX7 mRNA and protein in renal cancer tissues was significantly lower than that in paraneoplastic tissues (P<0.01) and correlated with lymph node metastasis and TNM grade (P<0.05). SOX7 mRNA expression in three renal cancer cells (A498, ACHN, 786 O) was significantly lower than that in the control group HK-2 (P<0.01). SOX7 mRNA expression was increased after 5-Aza-dC/TSA treatment in all (P<0.01). MSP results showed that the methylation rate of SOX7 was significantly higher in kidney cancer tissues than that in paraneoplastic tissues (P<0.01) and correlated with TNM stage (P<0.05). Overexpression of SOX7 inhibited the proliferative activity, migration ability and invasive ability of ACHN. Conclusion:  SOX7 is lowly expressed in renal cell carcinoma, hypermethylation of SOX7 may be one of the molecular mechanisms of renal carcinoma histogenesis.