| 肝细胞Foxa2基因剔除小鼠的制备及鉴定 |
| |
| 引用本文： | 张莉萍,Fulmer James.肝细胞Foxa2基因剔除小鼠的制备及鉴定[J].重庆医学,2005,34(6):890-891. |
| |
| 作者姓名： | 张莉萍 Fulmer James |
| |
| 作者单位： | 重庆医科大学附属第二医院临床检验中心,400010;美国宾夕法尼亚大学医学院遗传系 |
| |
| 摘 要： | 目的制备肝细胞Foxa2基因特异性剔除的小鼠模型,并进行鉴定.方法采用CreloxP重组系统,在Cre重组酶的作用下,将小鼠肝细胞DNA上的Foxa2基因进行条件性的靶向剔除;并通过PCR和免疫荧光染色法进行鉴定.结果PCR在剔除型小鼠中未扩增出Foxa2基因的条带;同时定量分析也未测出其mRNA的表达;免疫荧光显示剔除型小鼠的Foxa2蛋白也为阴性.结论条件性基因剔除技术能成功地制备肝细胞Foxa2基因特异性剔除的小鼠模型.
|
| 关 键 词： | Foxa2基因 肝细胞 基因剔出 聚合酶联反应 |
| 文章编号： | 1671-8348(2005)06-0890-02 |
Generation and confirmation of Foxa2 gene knockout in hepatocyte in mice |
| |
| ZHANG Li-ping,Fulmer James.Generation and confirmation of Foxa2 gene knockout in hepatocyte in mice[J].Chongqing Medical Journal,2005,34(6):890-891. |
| |
| Authors: | ZHANG Li-ping Fulmer James |
| |
| Abstract: | s:Objective To generate Foxa2 loxP/loxPAFP.Cre mice and confirm the genetype.Methods Cre-loxP system was introduced to mediate targeting of the Foxa2 gene and to achieve hepatocyte specific expression of Cre; real-time PCR and immunofluorescence staining were used to confirm absence of Foxa2 in livers from Foxa2 loxP/loxPAFP.Cre mice.Results There is no band for Foxa2 by PCR, no Foxa2 mRNA expression by real-time PCR in Foxa2 loxP/loxPAFP.Cre mice.Conclusion Foxa2 is deleted efficiently in Foxa2 loxP/loxPAFP.Cre mice by conditional gene knockout. |
| |
| Keywords: | Foxa2 gene hepatocyte gene knockout |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
