| Wistar大鼠腹主动脉瘤模型的建立及改进 |
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| 引用本文： | 许文平,邱健,詹纯列,何柏身,马国亮,谢志泉.Wistar大鼠腹主动脉瘤模型的建立及改进[J].医学研究生学报,2009,22(4). |
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| 作者姓名： | 许文平 邱健 詹纯列 何柏身 马国亮 谢志泉 |
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| 作者单位： | 1. 驻香港部队医院,广东深圳,518048
2. 广州军区广州总医院心内科,广东广州,510010 |
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| 摘 要： | 目的:腹主动脉瘤(AAA)是一类死亡率极高的主动脉疾病.为研究AAA的发病机制及治疗措施,必须建立合适的病理模型.文中研究建立简便、迅速、稳定的Wistar大鼠AAA模型. 方法:10周龄雄性Wistar大鼠30只,随机分成实验组及对照组(各15只),实验组采用缩短弹力酶灌注时间的改进建模方法,对照组采用传统建模方法.14d后计算2组动物的存活率、腹主动脉瘤的形成率,制作腹主动脉石蜡及电镜切片,观察其形态学变化.结果 :对照组大鼠手术存活率为60%,存活大鼠AAA形成率为89%;实验组大鼠存活率为87%,存活大鼠AAA形成率为85%;两组大鼠均可见腹主动脉明显扩张,动脉壁变硬,部分区域可见内膜撕裂,中膜弹力纤维减少或消失,外膜炎性细胞浸润明显. 结论:改进后的30min弹力酶灌注法可用于AAA大鼠模型的制作,动物成活率及AAA形成率均较高.是一种简便、迅速、稳定的腹主动脉瘤Wistar大鼠建模方法.
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| 关 键 词： | 腹主动脉瘤 动物模型 |
A rat models of abdominal aortic aneurysms |
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| XU Wen-ping,QIU Jian,ZHAN Chun-lie,HE Bo-shen,MA Guo-liang,XIE Zhi-quan.A rat models of abdominal aortic aneurysms[J].Bulletin of Medical Postgraduate,2009,22(4). |
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| Authors: | XU Wen-ping QIU Jian ZHAN Chun-lie HE Bo-shen MA Guo-liang XIE Zhi-quan |
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| Institution: | XU Wen-ping1,QIU Jian2,ZHAN Chun-lie2,HE Bo-shen1,MA Guo-liang1,XIE Zhi-quan2(1.The Military Hospital of PLA in Hongkong,Shenzhen 518048,Guangdong,China,2.Department of Cardiology,Guangzhou General Hospital of Guangzhou Military Region,PLA,Guangzhou 510010,China) |
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| Abstract: | Objective: Abdominal aortic aneurysm(AAA) is a kind of abdominal aortic disorders with very high mortality.This paper reports the innovated methods for a simple,quick,stable and high-successed-rate model of abdominal aortic aneurysms(AAA).Methods: Wistar rats were divided into control group and improving method group,and AAA models were made with traditional and modified method respectively.The two methods were compared with formation and mortality.Results: Both of the methods may induce AAA.The mortality o... |
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| Keywords: | Abdominal aortic aneurysms Animal model |
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