结肠癌组织中Shh和Gli1基因的表达及临床意义

目的探讨Shh和Gli1基因在结肠癌组织中的表达及意义。方法应用RT-PCR方法和免疫组织化学对38例结肠癌手术切除的标本进行Shh和Gli1基因表达检测,评估Shh和Gli1基因表达与结肠癌临床病理的关系。结果癌组织的Shh和Gli1基因呈高表达,与癌旁组织比较,两者有显著性差异;Shh和Gli1基因表达强度与癌的分期密切相关,早期结肠癌的表达强度明显高于进展期癌。结论 Shh信号通路异常的活性状态有可能参与结肠癌的发生过程。Shh和Gli1基因检测对评估结肠癌的分期和预后具有重要意义。

Expression and Clinical Significance of Shh and Gli1 Gene in Colon Carcinoma Tissue

Objective To investigate the effect of Shh and Gli1 gene on the invasion,metastasis and prognosis of colon cancer.Methods Immunohistochemistry and RT-PCR method were used to evaluate the expression of Shh and Gli1 gene,and the relationship between its expression and the clinicopathological prognostic parameters was analyzed in 38 cades of colon cancer.Results There were high expression of Shh and Gli1 gene in colon cancer,the expression of Shh and Gli1 gene in colon cancer was elevated compare with their corresponding adjacent benign tissues.The expression of Shh and Gli1 gene in colon cancer was closly correlated to cancer staging,the expression of Shh and Gli1 gene in early-stage colon cancer was higher than that in advanced stage.Conclusion Abnormal active state of Shh signaling pathway maybe participate in the incidence of colon cancer,the detection of Shh and Gli1 gene take an important role in the assessment of colon cancer staging and prognosis.