奥曲肽对人胃癌细胞的凋亡诱导作用

目的 探讨生长抑素对人胃癌细胞的抑制作用及其作用机理。方法 以生长抑素类似物奥曲肽作用于人胃癌细胞株SGC-7901细胞，以人成纤维细胞株HF作为正常细胞对照，以5-FU作为阳性药物对照，通过MTT实验、荧光显微镜观察及流式细胞术分析，得出结论。结果 一定浓度范围内的奥曲肽对SGC-7901细胞产生抑制作用，其抑制作用具有量效关系和饱和性。奥曲肽对SGC-7901细胞的抑制程度接近于5-FU对他的抑制，而对HF细胞无影响。奥曲肽可诱导SGC-7901细胞凋亡。结论 奥曲肽在体外对SGC-7901细胞具有抑制作用，诱导肿瘤细胞凋亡可能是其作用机理之一。

Apoptosis-Induction Effects of Octreotide on Human Gastric Cancer Cells

Objective To study the effects and mechanism of Octreotide to inhibit the proliferation of human gastric cancer cells in vitro . Methods Human gastric cancer cell line SGC 7901 was treated with Octreotide. Human fibroblast cell line HF and 5 FU were used as control. MTT assay and fluorescent microscopy as well as flow cytometry were performed in this study. Results Octreotide inhibited the growth of SGC 7901 in vitro within certain concentrations. The suppression was quantity dependent but did not occur when up to a certain concentration. There was no difference between Octreotide and 5 FU in their inhibition on SGC 7901. Octreotide had no effects on normal human fibroblast cell line HF. When SGC 7901 was treated with Octreotide, the typical apoptotic bodies were identified by flow cytometry and fluorescent microscopy. Conclusion Octreotide can inhibit the proliferation of human gastric cancer cell line SGC 7901 in vitro . The induction of apoptosis by Octreotide might be the primary mechanism.