| 人胃癌细胞乙酰肝素酶对Src激酶活化的影响 |
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| 引用本文： | 李连兴,韩利芳,马秀梅. 人胃癌细胞乙酰肝素酶对Src激酶活化的影响[J]. 肿瘤防治研究, 2012, 39(3): 256-259. DOI: 10.3971/j.issn.1000-8578.2012.03.003 |
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| 作者姓名： | 李连兴 韩利芳 马秀梅 |
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| 作者单位： | 1.010059 呼和浩特，内蒙古医学院附属医院病理科 内蒙古医学院基础医学院病理学教研室，2.生理学教研室 |
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| 基金项目： | 国家自然科学基金资助项目(30660065);教育部春晖计划资助项目(Z2007—1-01003);博士科研启动资金资助项目(NY2006BQ006) |
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| 摘 要： | 目的探讨人胃癌细胞乙酰肝素酶与Src激酶的关系。方法利用Western blot检测Src激酶抑制剂pp2的活性、pp2对人胃癌SGC-7901细胞中乙酰肝素酶表达的影响以及乙酰肝素酶表达被沉默的人胃癌SGC-7901细胞中磷酸化Src（p-Src）激酶蛋白的表达。结果pp2对乙酰肝素酶蛋白表达无影响，在乙酰肝素酶被沉默的人胃癌SGC-7901细胞中p-Src蛋白表达降低。结论人胃癌细胞乙酰肝素酶可能调节Src激酶的磷酸化，乙酰肝素酶-Src可能是参与人胃癌侵袭、迁移和诱导血管生成的一条信号通路。
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| 关 键 词： | 胃癌 乙酰肝素酶 Src激酶 |
| 收稿时间： | 2011-08-26 |
Effect of Heparanase on Src Kinases Activation in Human Gastric Carcinoma Cells |
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| Li lianxing , Han Lifang , Ma Xiumei. Effect of Heparanase on Src Kinases Activation in Human Gastric Carcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2012, 39(3): 256-259. DOI: 10.3971/j.issn.1000-8578.2012.03.003 |
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| Authors: | Li lianxing Han Lifang Ma Xiumei |
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| Affiliation: | 1.Department of Pathology，The Affiliated Hospital of Inner Mongolia Medical College Department of Pathology，School of Basic Medical Science of Inner Mongolia Medical College，Huhhot 010059，China，2.Department of Physiology |
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| Abstract: | Objective To investigate the relationship of heparanase and Src in gastric carcinoma cells.Methods Western-blot was used to detect pp2 activity,the inhibitor of Src,and its effect on heparanase expression of human gastric carcinoma SGC-7901 cells,and p-Src expression in human gastric carcinoma SGC-7901 cells with silenced Heparanase.Results pp2,the inhibitor of Src,did not affect Heparanase protein expression in human gastric carcinoma SGC-7901 cells,p-Src expression decreased in human gastric carcinoma SGC-7901 cells with silenced Heparanase.Conclusion Heparanase might regulate Src phosphorylation,and Heparanase-Src might be a signal transduction pathway of invasion,migration and angiongenesis of human gastric cancer. |
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| Keywords: | Stomach neoplasms Heparanase Src |
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