诱导痰技术在检测哮喘气道炎症中的应用

目的：探讨诱导痰技术在哮喘气道炎症检测方面的应用价值。方法：对12例正常人,11例缓解期哮喘患者及10例急性发作期哮喘患者进行痰液诱导测试,并对受试者血浆及诱导痰白细胞介素－8（IL－8）、嗜酸细胞阳离子蛋白（ECP）、丙二醛（MDA）水平进行测定比较。结果：33例受试者中32例完成了测试;急性发作期哮喘患者的血浆及诱导痰IL－8水平、ECP水平、MDA水平与缓解期哮喘患者相比显著增高（P均小于0．01）：而缓解期哮喘患者的血浆及诱导痰IL－8、ECP、MDA水平仍明显高于正常志愿者（P均小于0．05）;且哮喘患者诱导痰的IL－8、ECP、MDA水平明显高于其血浆的IL－8（P＜0．05）、ECP（P＜0．01）、MDA（P＜0．01）水平。结论：诱导痰技术安全、可靠;诱导痰IL－8、ECP、MDA水平可用于监测哮喘气道炎症。

Clinical value of induced sputum test in monitoring airway inflammation in asthma

Objective To explore the clinical value of induced sputum test in monitoring airway inflammation in asthmatic patients.Methods The induced sputum test was done in12nomal subjects and21as-thmatic subjects;The levels of interleukin-8(IL-8),eosinophil cation protein(ECP),malondialdehyde(MDA)in plasma and induced sputum in all subjects were measured.Results The levels of IL-8,ECP,MDA in plasma and induced sputum in unstable asthmatic patients were significantly higher than those in stable asthmatic(P<0.01),in which the levels of IL-8,ECP,MDA in both plasma and induced sputum were significantly higher than those in nomal subjects(P<0.05);In all asthmatic patients,the levels of IL-8,ECP,MDA in induced sputum were significanty higher than those in plasma.Conclusion The levels of IL-8,ECP,MDA in induced sputum could be used for monitoring airway inflammation in asthmatic patients.