Cytosolic Ca2+ under high glucose with suppressed Na+/K+ pump activity in rat sensory neurons |
| |
Authors: | Sanada Mitsuru Matsuura Hiroshi Omatsu-Kanbe Mariko Sango Kazunori Kashiwagi Atsunori Yasuda Hitoshi |
| |
Affiliation: | Division of Neurology, Department of Medicine, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan. |
| |
Abstract: | Cytosolic Ca2+ concentration ([Ca2+]i) was measured in isolated rat dorsal root ganglion (DRG) neurons using the fluorescent Ca2+ indicator fura-2. Exposure to high (50 mM) extracellular K+ evoked a robust increase in [Ca2+]i, which was almost totally abolished by concomitant presence of nisoldipine (10 microM) and omega-conotoxin GVIA (10 microM). Whereas either high (30 mM) D-glucose alone or ouabain (100 microM) alone did not appreciably affect the high K+-induced [Ca2+]i elevation, neurons pretreated with high D-glucose together with ouabain exhibited a significantly larger [Ca2+]i response to high K+ stimulation, which was almost completely inhibited by nisoldipine and omega-conotoxin GVIA. These results suggest that a combination of high glucose and suppressed Na+/K+ pump activity potentiates the [Ca2+]i elevation stimulated by activation of the voltage-gated Ca2+ channels in rat DRG neurons. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|