补肾化痰祛瘀法对血管性痴呆大鼠认知功能的保护作用

目的研究补肾化痰祛瘀方对血管性痴呆（VaD）大鼠认知功能的改善作用。方法在缺糖缺氧培养条件下，观察补肾化痰祛瘀方血清对体外培养的海马神经元的保护作用。用双侧颈总动脉永久性结扎法（2VO）制备VaD大鼠模型，造模前予灌胃治疗，Morris水迷宫测试治疗后VaD大鼠认知功能的改善情况，TUNEL染色测定各组大鼠海马脑组织内细胞凋亡的变化。免疫组织化学方法检测Bcl-2、Bax蛋白表达。结果在缺糖缺氧培养条件下，体外培养海马神经元出现大量的细胞凋亡，而预先加入补肾化痰祛瘀方的血清进行处理后，细胞凋亡率明显下降。补。肾化痰祛瘀方灌胃治疗的VaD大鼠，与未予治疗的VaD大鼠组相比，治疗组大鼠找到平台的潜伏期缩短（P〈0．01），穿越平台次数增多（P〈0．01）；与痴呆组相比，补肾化痰祛瘀方治疗组大鼠脑组织海马区凋亡细胞数明显减少，抗凋亡基因Bcl-2蛋白表达显著增加，凋亡基因Bax蛋白表达降低，差异有统计学意义（P〈0．01）。结论补肾化痰祛瘀方对大鼠体外培养和脑组织内海马神经元具有神经保护作用，补肾化痰祛瘀方治疗可以明显改善VaD大鼠的认知功能。

Study of invigorating kidney, dissipating phlegm and resolving stasis recipe on cognitive function improvement of rats with vascular dementia

Objective To study improvement of cognitive function impairment of vascular dementia rats induced by a permanent bilateral ligation of common carotid arteries （2VO） after administration of invigorating kidney, dissipating phlegm and resolving stasis recipe. Methods Hippocampal neurons was isolated and cultured from SD rats. At oxy- gen-glucose deprivation condition, protection role of invigorating kidney, dissipating phlegm and resolving stasis recipe on hippocampal neurons was observed. Animal model of rats was established by 2VO, Pre-treatment VaD model was established by administration of invigorating kidney, dissipating phlegm and resolving stasis recipe. Morris water test was performed to detect difference in the cognitive function between VaD group and control group. Neuron apoptosis and expression of Bcl-2 protein and Bax protein in hippocampus tissue were detected. Results Plenty of apoptotic cells were seen in hippocampal neurons after oxygen-glucose deprivation cultured, while apoptosis rate was much de- creased in treatment group. Compared leptin treatment group with control group, difference has a statistical signifi- cance （P 〈 0.01 ）. Compared with untreated VaD group, learning and memory ability was improved in treatment VaD group. Neuron apoptosis in hippocampus tissue of treated group were different significantly from those of untreated group （P 〈 0.01）. Conclusion Invigorating kidney, dissipating phlegm and resolving stasis recipe could protect hippocam- pal neurons from apoptosis in vitro. Cognitive function impairment could be improved by administration of invigorating kidney, dissipating phlegm and resolving stasis recipe in VaD rats.