miR-497 靶向Bcl-2 调控肝癌细胞增殖及凋亡的研究 |
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引用本文: | 张猛,张全,谭雨莎.miR-497 靶向Bcl-2 调控肝癌细胞增殖及凋亡的研究[J].中国肿瘤临床,2016,43(16):697-701. |
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作者姓名: | 张猛 张全 谭雨莎 |
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作者单位: | 作者单位:河北大学附属医院肝胆外科(河北省保定市071000) |
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摘 要: |
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关 键 词: | miR-497? Bcl- 2? 肝癌 细胞增殖 细胞凋亡 |
收稿时间: | 2016-04-05 |
miR-497 regulates cell proliferation and apoptosis by targeting Bcl-2 in liver cancer cells |
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Institution: | Department of Hepatobiliary Surgery of Affiliated Hospital of Hebei University, Baoding071000, China |
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Abstract: | Objective:To evaluate the effect of miR-497 on regulating cell proliferation and apoptosis by targeting Bcl-2 in liver cancer cells. Methods:We tested liver cancer tissue and para-carcinoma tissue and used RT-PCR or Western blot to detect the expression of miR-497and Bcl-2 protein. We also tested the liver cancer cel HepG2 transfected with miR-497 mimics and mimic control. The expressions of miR-497and Bcl-2 protein were detected by RT-PCR or Western blot. Cell proliferation activity was detected by the MTT method, cell apoptosis was detected by flow cytometry, and cel luciferase activity was detected by the dual-luciferase reporter gene experiment. Results:1) Compared with para-carcinoma tissue, the miR-497 expression of liver cancer tissue significantly decreased (P<0.05), whereas the Bcl-2 protein expression of liver cancer tissue significantly increased (P<0.05). 2) Compared with transfection mimic control, transfection miR-497 mimics could increase the miR-497 expression of liver cancer cel HepG2 (P<0.05) and decrease the Bcl-2 protein expression of liver cancer cel HepG2 (P<0.05). 3) Compared with transfection mimic control, co-transfection with miR-497 mimics and Bcl-2-WT could significantly decrease the luciferase activity of liver cancer cell HepG2 (P<0.05). 4) Compared with transfection mimic control, the proliferative activity of liver cancer cell HepG2 significantly decreased after transfection with miR-497 mimics (P<0.05). Compared with transfection miR-497 mimics, the proliferative activity of liver cancer cell HepG2 significantly increased after transfection with miR-497 mimics+Bcl-2 (P<0.05). 5) Compared with transfection mimic control, the total apoptosis rate of liver cancer cell HepG2 significantly increased after transfection with miR-497 mimics (P<0.05). Compared with transfection miR-497 mimics, the total apoptosis rate of liver cancer cell HepG2 significantly decreased after transfection with miR-497 mimics+Bcl-2 (P<0.05). Conclusion:In liver cancer cel s, miR-497 could target Bcl-2 to inhibit cel proliferation and enhance cell apoptosis. |
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Keywords: | miR-497 Bcl-2 liver cancer cell proliferation cell apoptosis |
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