vMAlteration of bile acid metabolism in pseudo germ-free rats |
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Authors: | Salil Kumar Bhowmik Ji Hye An Soo Hyun Lee Byung Hwa Jung |
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Institution: | 1. Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 136-791, Korea 2. University of Science and Technology, Daejeon, 305-350, Korea 3. Division of Life and Pharmaceutical Science and College of Pharmacy, Ewha Woman??s University, Seoul, 120-750, Korea 4. Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 130-650, Korea
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Abstract: | To characterize the impact of gut microbiota on host bile acid metabolism, we investigated the metabolic profiles of oxysterols and bile acids (BAs) in a conventional rat model (SD) (n=5) and its pseudo germ-free (GF) equivalent (n=5). GF rats were developed by the oral administration of bacitracin, neomycin and streptomycin (200 mg/kg, each) twice a day for 6 days. Urinary levels of oxysterols and bile acid metabolites were quantified using gas chromatography-mass spectrometry (GC-MS). The activity levels of enzymes involved in the bile acid metabolic pathway were determined through urinary concentration ratio between product to precursor. Cholic acid (CA) and ??-/??-muricholic acid (??-/??-MCA) were significantly elevated at pseudo germ-free condition. An increase of hydroxylase (cholesterol 7??-hydroxylase, oxysterol 7??-hydroxylase and cytochrome P450 scc) and a significant decrease of 7??-dehydroxylase were observed. The urinary concentration ratio of primary bile acids, a marker for hepatotoxicity, increased in pseudo germfree conditions. Therefore, it was found that gut microbiota could play a significant role in the bile acids homeostasis and metabolism. |
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